6C8-A2

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$40.00
SKU: 6C8-A2
View product citations for antibody 6C8-A2 on CiteAb

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Available: 79

DSHB Data Sheet

Catalog Fields

Antigen: Regenerating blastema; intestinal cells
Hybridoma Cells Available: Yes
Antigen Species: Schmidtea mediterranea
Depositor: Zayas, R.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Biology, San Diego State University
Positive Tested Species Reactivity: Schmidtea mediterranea
Depositors Notes: Nuclear staining in intact planarians. Specific to a population of intestinal cells.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Formaldehyde-fixed cells from regenerating blastema of C1W4 clonal lineage
Alternate Gene Names:
Alternate Antibody Name: 6C8
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63Ag8U.1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2619612 
Additional Information:
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6C8-A2 was deposited to the DSHB by Zayas, R.M. (DSHB Hybridoma Product 6C8-A2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • All References
  • Initial Publication

    Novel monoclonal antibodies to study tissue regeneration in planarians.
    Zayas RM
    BMC developmental biology 15. (2015 Jan 21): 2.

    IF References

    Novel monoclonal antibodies to study tissue regeneration in planarians.
    Zayas RM
    BMC developmental biology 15. (2015 Jan 21): 2.

    All References

    Novel monoclonal antibodies to study tissue regeneration in planarians.
    Zayas RM
    BMC developmental biology 15. (2015 Jan 21): 2.

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