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SKU: 6D1-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 6D1
Available to For-Profits: Yes
Alternate Antibody Name: CD42b
Gene Name: GP1BA
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2617391 
Uniprot ID: P07359 
Entrez Gene ID: 2811 
Clonality: Monoclonal
Immunogen: Platelets
Immunogen Sequence: Full length sequence
Myeloma Strain: X63-Ag8.653
Epitope Mapped: Yes
Antigen Name: Platelet glycoprotein Ib alpha chain; CD42b-alpha
Epitope Location or Sequence: Near the vWf binding site.
Alternate Antigen Name:
Deposit Date: 3/3/2012
Antigen Molecular Weight: 71.5 kDa
Depositor: Coller, B.S.
Antigen Sequence:
Depositor Institution: Rockefeller University
Antigen Species: Human
Depositor Notes: This antibody blocks the binding of GP-1b to the A1 domain of Von Willebrand Factor (vWF).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human, Primate
Additional Information: RRID:AB_2617391
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6D1 was deposited to the DSHB by Coller, B.S. (DSHB Hybridoma Product 6D1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

11 References

  • Initial Publication
  • IF References
  • IP References
  • ELISA References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IP References
    ELISA References
    FB References
    FACS References
    Epitope Map References
    All References

    Determination of platelet antigens and glycoproteins in the human fetus.
    Wautier JL
    Blood 68.2 (1986 Aug): 488-92.

    A murine monoclonal antibody that completely blocks the binding of fibrinogen to platelets produces a thrombasthenic-like state in normal platelets and binds to glycoproteins IIb and/or IIIa.
    Sullivan CA
    The Journal of clinical investigation 72.1 (1983 Jul): 325-38.

    Partial characterization of a binding site for von Willebrand factor on glycocalicin.
    Handin RI
    Blood 67.1 (1986 Jan): 19-26.

    Identification of platelet glycoprotein IIb/IIIa as the major binding site for released platelet-von Willebrand factor.
    Gralnick HR
    Blood 68.3 (1986 Sep): 732-6.

    Platelet membrane alterations induced by the local anesthetic dibucaine.
    Peerschke EI
    Blood 68.2 (1986 Aug): 463-71.

    Identification of the thrombin receptor on human platelets by chemical crosslinking.
    Gralnick HR
    The Journal of clinical investigation 77.2 (1986 Feb): 362-8.

    von Willebrand factor is present on the surface of platelets stimulated in plasma by ADP.
    Powers P
    Blood 70.5 (1987 Nov): 1362-6.

    Monoclonal antibodies to the glycoprotein IIb-IIIa epitopes involved in adhesive protein binding: effects on platelet spreading and ultrastructure on human arterial subendothelium.
    Gralnick HR
    The Journal of laboratory and clinical medicine 109.4 (1987 Apr): 495-503.

    Role of adhesive proteins in platelet tumor interaction in vitro and metastasis formation in vivo.
    Coller BS
    The Journal of clinical investigation 81.4 (1988 Apr): 1012-9.

    Evaluation of platelet glycoprotein Ib by fluorescence flow cytometry.
    Ault KA
    Blood 66.2 (1985 Aug): 423-7.

    Plasminogen interactions with platelets in plasma.
    Hanners E
    Blood 72.5 (1988 Nov): 1530-5.

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