6D1

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$40.00
SKU: 6D1-s
View product citations for antibody 6D1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Platelet glycoprotein Ib alpha chain; CD42b-alpha
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Coller, B.S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Rockefeller University
Positive Tested Species Reactivity: Human, Primate
Depositors Notes: This antibody blocks the binding of GP-1b to the A1 domain of Von Willebrand Factor (vWF).
Antigen Molecular Weight: 71.5 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: GP1BA
Immunogen: Platelets
Alternate Gene Names: CD42b, GP1B, GPIbalpha
Alternate Antibody Name: CD42b
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: X63-Ag8.653
Epitope Location or Sequence: Near the vWf binding site.
Uniprot ID: P07359 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 2811 
Additional Characterization:
Antibody Registry ID: AB_2617391 
Additional Information: RRID:AB_2617391
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6D1 was deposited to the DSHB by Coller, B.S. (DSHB Hybridoma Product 6D1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • IP References
  • ELISA References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    The Journal of clinical investigation
    Sullivan CA
    The Journal of clinical investigation 72.1 (1983 Jul): 325-38.

    IF References

    Blood
    Wautier JL
    Blood 68.2 (1986 Aug): 488-92.

    IP References

    The Journal of clinical investigation
    Sullivan CA
    The Journal of clinical investigation 72.1 (1983 Jul): 325-38.

    ELISA References

    Blood
    Handin RI
    Blood 67.1 (1986 Jan): 19-26.

    FB References

    Blood
    Handin RI
    Blood 67.1 (1986 Jan): 19-26.

    Blood
    Wautier JL
    Blood 68.2 (1986 Aug): 488-92.

    Blood
    Gralnick HR
    Blood 68.3 (1986 Sep): 732-6.

    Blood
    Peerschke EI
    Blood 68.2 (1986 Aug): 463-71.

    The Journal of clinical investigation
    Gralnick HR
    The Journal of clinical investigation 77.2 (1986 Feb): 362-8.

    Blood
    Powers P
    Blood 70.5 (1987 Nov): 1362-6.

    The Journal of laboratory and clinical medicine
    Gralnick HR
    The Journal of laboratory and clinical medicine 109.4 (1987 Apr): 495-503.

    The Journal of clinical investigation
    Coller BS
    The Journal of clinical investigation 81.4 (1988 Apr): 1012-9.

    FACS References

    Blood
    Ault KA
    Blood 66.2 (1985 Aug): 423-7.

    Blood
    Wautier JL
    Blood 68.2 (1986 Aug): 488-92.

    Blood
    Hanners E
    Blood 72.5 (1988 Nov): 1530-5.

    Epitope Map References

    Blood
    Handin RI
    Blood 67.1 (1986 Jan): 19-26.

    All References

    Blood
    Wautier JL
    Blood 68.2 (1986 Aug): 488-92.

    The Journal of clinical investigation
    Sullivan CA
    The Journal of clinical investigation 72.1 (1983 Jul): 325-38.

    Blood
    Handin RI
    Blood 67.1 (1986 Jan): 19-26.

    Blood
    Gralnick HR
    Blood 68.3 (1986 Sep): 732-6.

    Blood
    Peerschke EI
    Blood 68.2 (1986 Aug): 463-71.

    The Journal of clinical investigation
    Gralnick HR
    The Journal of clinical investigation 77.2 (1986 Feb): 362-8.

    Blood
    Powers P
    Blood 70.5 (1987 Nov): 1362-6.

    The Journal of laboratory and clinical medicine
    Gralnick HR
    The Journal of laboratory and clinical medicine 109.4 (1987 Apr): 495-503.

    The Journal of clinical investigation
    Coller BS
    The Journal of clinical investigation 81.4 (1988 Apr): 1012-9.

    Blood
    Ault KA
    Blood 66.2 (1985 Aug): 423-7.

    Blood
    Hanners E
    Blood 72.5 (1988 Nov): 1530-5.

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