6F1
Catalog Fields
Product Name/ID: 6F1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: ITGA2
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2617392
Uniprot ID: P17301
Entrez Gene ID: 3673
Clonality: Monoclonal
Immunogen: Human platelets
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: X63-Ag8.653
Epitope Mapped: Yes
Antigen Name: integrin alpha-2 (CD49B), human
Epitope Location or Sequence: I domain of alpha2 (aa 173-259)
Alternate Antigen Name:
Deposit Date: 3/3/2012
Antigen Molecular Weight: Predicted: 129 kDa; Apparent: 154 kDa under non-reducing conditions, 166 kDa under reducing conditions.
Depositor: Coller, B.S.
Antigen Sequence:
Depositor Institution: Rockefeller University
Antigen Species: Human
Depositor Notes: Inhibits binding of collagen to integrin alpha2beta1. This antibody doesn't appear to work for western blotting.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human, Primate
Additional Information: 6F1 recognizes alpha2 in its native conformation associated to beta1 integrin.
Recommended Applications: ELISA, FACS, Function Blocking, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6F1 was deposited to the DSHB by Coller, B.S. (DSHB Hybridoma Product 6F1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
24 References
Initial Publication