6F1
Catalog Fields
Antigen: integrin alpha-2 (CD49B), human
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Coller, B.S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Rockefeller University
Positive Tested Species Reactivity: Human, Primate
Depositors Notes: Inhibits binding of collagen to integrin alpha2beta1. This antibody doesn't appear to work for western blotting.
Antigen Molecular Weight: Predicted: 129 kDa; Apparent: 154 kDa under non-reducing conditions, 166 kDa under reducing conditions.
Human Protein Atlas:
Predicted Species Reactivity:
Gene: ITGA2
Immunogen: Human platelets
Alternate Gene Names: CD49B, GPIA, BR, HPA-5, VLA-2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: X63-Ag8.653
Epitope Location or Sequence: I domain of alpha2 (aa 173-259)
Uniprot ID: P17301
Immunogen Sequence: Full length protein
Entrez Gene ID: 3673
Additional Characterization:
Antibody Registry ID: AB_2617392
Additional Information: 6F1 recognizes alpha2 in its native conformation associated to beta1 integrin.
Recommended Applications: ELISA, FACS, Function Blocking, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6F1 was deposited to the DSHB by Coller, B.S. (DSHB Hybridoma Product 6F1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
24 References
Initial Publication