6F1
Catalog Fields
Antigen: integrin alpha-2 (CD49B), human
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Coller, B.S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Rockefeller University
Positive Tested Species Reactivity: Human, Primate
Depositors Notes: Inhibits binding of collagen to integrin alpha2beta1. This antibody doesn't appear to work for western blotting.
Antigen Molecular Weight: Predicted: 129 kDa; Apparent: 154 kDa under non-reducing conditions, 166 kDa under reducing conditions.
Predicted Species Reactivity:
Gene: ITGA2
Immunogen: Human platelets
Alternate Gene Names: CD49B, GPIA, BR, HPA-5, VLA-2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: X63-Ag8.653
Epitope Location or Sequence: I domain of alpha2 (aa 173-259)
Uniprot ID: P17301
Immunogen Sequence: Full length protein
Entrez Gene ID: 3673
Antibody Registry ID: AB_2617392
Additional Information: 6F1 recognizes alpha2 in its native conformation associated to beta1 integrin.
Recommended Applications: ELISA, FACS, Function Blocking, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6F1 was deposited to the DSHB by Coller, B.S. (DSHB Hybridoma Product 6F1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
24 References
Initial Publication