6H1-Xi/Rabbit
Catalog Fields
Clone ID/Product Name: 6H1-Xi/Rabbit
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: MYH1
Ab Isotype: Rabbit IgG
Gene Name:
Antibody Registry ID: AB_3696610
Uniprot ID: Q9TSE5
Entrez Gene ID: 100125991
Clonality: Monoclonal
Immunogen: Retractor bulbi (adult rabbit) crude muscle extract muscle co-absorbed with adjuvant peptide to gold particles as immunogen carrier.
Clone:
Immunogen Sequence:
Myeloma Strain:
Epitope Mapped: No
Antigen Name: Myosin heavy chain, Type IIX
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/15/2025
Antigen Molecular Weight: ~200 kDa
Depositor: DSHB
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: rabbit
Depositor Notes: This is a chimeric recombinant rabbit IgG antibody. The VH and VL sequences were obtained from the 6H1 hybridoma cells, which produce mouse IgM antibody.
Host Species: rabbit
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Feline, Guinea Pig, Human, Mouse, Rabbit, Rat
Additional Information: 6H1 has been successfully used to stain skeletal muscle paraffin sections. Low pH antigen retrieval and pre-treatment with Proteinase K is required. See PUBMED ID: 24924099.
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6H1-Xi/Rabbit was deposited to the DSHB by DSHB (DSHB Hybridoma Product 6H1-Xi/Rabbit)
Storage and Handling Recommendations
Although many cell products are maintained at 4?C for years without loss of activity, shelf-life at 4?C is highly variable. For immediate use, short term storage at 4?C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 µl for freezing at -20?C or -80?C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 µg/ml. For western blots, the recommended concentration range is 0.2-0.5 µg/ml.
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