7-2C12

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$40.00
SKU: 7-2C12
View product citations for antibody 7-2C12 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Collagen, type I
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: De Blas, Angel L.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Connecticut
Positive Tested Species Reactivity: Rat
Depositors Notes:
Antigen Molecular Weight: 137.9 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Col1a1
Immunogen: Synaptosomal plasma membrane fraction from cerebral cortex
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Location or Sequence:
Uniprot ID: P02454 
Immunogen Sequence: Full length protein
Entrez Gene ID: 29393 
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: ELISA, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7-2C12 was deposited to the DSHB by De Blas, Angel L. (DSHB Hybridoma Product 7-2C12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • All References
  • Initial Publication

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    WB References

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    IHC References

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    IP References

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    ELISA References

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    All References

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

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