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SKU: 76-E6-s

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 76-E6
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: Hspa5
Ab Isotype: RIgG2, kappa light chain
Gene Name:
Antibody Registry ID: AB_2795268 
Uniprot ID: P20029 
Entrez Gene ID: 14828 
Clonality: Monoclonal
Immunogen: BiP:mu chain complexes
Immunogen Sequence:
Myeloma Strain: P3X63.Ag8.653
Epitope Mapped: Yes
Antigen Name: BiP (GRP78)/ Endoplasmic reticulum chaperone BiP
Epitope Location or Sequence: a.a. 497-581
Alternate Antigen Name:
Deposit Date: 9/21/2023
Antigen Molecular Weight: 72.4 kDa
Depositor: UAB Research Foundation
Antigen Sequence:
Depositor Institution: U of Alabama, Birmingham
Antigen Species: mouse
Depositor Notes: BiP is a member of the hsp70 family. BiP is found the endoplasmic reticulum and binds to free intracellular Ig heavy chains in the absence of Ig light chains. Hybridoma developed by J.F.Kearney.
Host Species: rat
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Mouse
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
76-E6 was deposited to the DSHB by UAB Research Foundation (DSHB Hybridoma Product 76-E6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

3 References

  • Initial Publication
  • WB References
  • IP References
  • ELISA References
  • All References
  • Initial Publication
    WB References
    IP References
    ELISA References
    All References

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