7E2
Catalog Fields
Antigen: integrin beta-1 /CD29
Hybridoma Cells Available: Yes
Antigen Species: Chinese hamster
Depositor: Juliano, R.L.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: UNC - Chapel Hill
Positive Tested Species Reactivity: Chinese Hamster
Depositors Notes: This antibody recognizes a confirmational epitope on the beta subunit that is dependent upon disulfide bonding. 7E2 fails to recognize B1 integrin by immunoblot on WISH, HDF, BHK, NRK and NIH 3T3 cells.
Antigen Molecular Weight: 110 kDa (native)
Human Protein Atlas:
Predicted Species Reactivity:
Gene: I79_016350
Immunogen: Purified fibronectin receptor from CHO cells
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P2x63.Ag8.63
Epitope Location or Sequence:
Uniprot ID: G3HZ55
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_532374
Additional Information: RRID:AB_532374
Recommended Applications: FACS, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7E2 was deposited to the DSHB by Juliano, R.L. (DSHB Hybridoma Product 7E2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
11 References
Initial Publication
Monoclonal antibodies to distinctive epitopes on the alpha and beta subunits of the fibronectin receptor.
Juliano RL
Experimental cell research 177.2 (1988 Aug): 303-18.