7E2
Catalog Fields
Antigen: integrin beta-1 /CD29
Hybridoma Cells Available: Yes
Antigen Species: Chinese hamster
Depositor: Juliano, R.L.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: UNC - Chapel Hill
Positive Tested Species Reactivity: Chinese Hamster
Depositors Notes: This antibody recognizes a confirmational epitope on the beta subunit that is dependent upon disulfide bonding. 7E2 fails to recognize B1 integrin by immunoblot on WISH, HDF, BHK, NRK and NIH 3T3 cells.
Predicted Species Reactivity:
Gene: I79_016350
Immunogen: Purified fibronectin receptor from CHO cells
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P2x63.Ag8.63
Epitope Location or Sequence:
Uniprot ID: G3HZ55
Immunogen Sequence:
Antibody Registry ID: AB_532374
Additional Information: RRID:AB_532374
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7E2 was deposited to the DSHB by Juliano, R.L. (DSHB Hybridoma Product 7E2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
12 References
Initial Publication
Monoclonal antibodies to distinctive epitopes on the alpha and beta subunits of the fibronectin receptor.
Juliano RL
Experimental cell research 177.2 (1988 Aug): 303-18.