7E2

(0) No Reviews yet
$40.00
SKU: 7E2
View product citations for antibody 7E2 on CiteAb

In Stock

Available: 179

DSHB Data Sheet

Catalog Fields

Antigen: integrin beta-1 /CD29
Hybridoma Cells Available: Yes
Antigen Species: Chinese hamster
Depositor: Juliano, R.L.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: UNC - Chapel Hill
Positive Tested Species Reactivity: Chinese Hamster
Depositors Notes: This antibody recognizes a confirmational epitope on the beta subunit that is dependent upon disulfide bonding. 7E2 fails to recognize B1 integrin by immunoblot on WISH, HDF, BHK, NRK and NIH 3T3 cells.
Antigen Molecular Weight: 110 kDa (native)
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: I79_016350
Immunogen: Purified fibronectin receptor from CHO cells
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P2x63.Ag8.63
Epitope Location or Sequence:
Uniprot ID: G3HZ55 
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_532374 
Additional Information: RRID:AB_532374
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7E2 was deposited to the DSHB by Juliano, R.L. (DSHB Hybridoma Product 7E2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • FB References
  • FACS References
  • All References
  • Initial Publication

    Experimental cell research
    Juliano RL
    Experimental cell research 177.2 (1988 Aug): 303-18.

    IF References

    Blood
    Jennings LK
    Blood 100.13 (2002 Dec 15): 4502-11.

    The Journal of cell biology
    Horwitz AF
    The Journal of cell biology 153.7 (2001 Jun 25): 1427-40.

    The Journal of biological chemistry
    Gu J
    The Journal of biological chemistry 281.44 (2006 Nov 3): 33258-67.

    Experimental cell research
    Parrilla R
    Experimental cell research 314.10 (2008 Jun 10): 2004-15.

    Experimental cell research
    Kuentzel SL
    Experimental cell research 184.2 (1989 Oct): 407-26.

    WB References

    Experimental cell research
    Juliano RL
    Experimental cell research 177.2 (1988 Aug): 303-18.

    The Journal of biological chemistry
    Gu J
    The Journal of biological chemistry 290.49 (2015 Dec 4): 29345-60.

    Experimental cell research
    Parrilla R
    Experimental cell research 314.10 (2008 Jun 10): 2004-15.

    Experimental cell research
    Kuentzel SL
    Experimental cell research 184.2 (1989 Oct): 407-26.

    IP References

    Experimental cell research
    Juliano RL
    Experimental cell research 177.2 (1988 Aug): 303-18.

    Blood
    Jennings LK
    Blood 100.13 (2002 Dec 15): 4502-11.

    The Journal of biological chemistry
    Gu J
    The Journal of biological chemistry 281.44 (2006 Nov 3): 33258-67.

    Experimental cell research
    Kuentzel SL
    Experimental cell research 184.2 (1989 Oct): 407-26.

    ELISA References

    Experimental cell research
    Kuentzel SL
    Experimental cell research 184.2 (1989 Oct): 407-26.

    FB References

    mAbs
    Soll DR
    mAbs 11.4 (2019 May/Jun): 691-708.

    FACS References

    FEBS letters
    Wewer UM
    FEBS letters 579.25 (2005 Oct 24): 5589-95.

    Journal of cell science
    Woods A
    Journal of cell science 113 ( Pt 3). (2000 Feb): 493-506.

    The Journal of cell biology
    Languino LR
    The Journal of cell biology 166.3 (2004 Aug 2): 407-18.

    Blood
    Miyata T
    Blood 113.21 (2009 May 21): 5304-13.

    All References

    Blood
    Jennings LK
    Blood 100.13 (2002 Dec 15): 4502-11.

    The Journal of cell biology
    Horwitz AF
    The Journal of cell biology 153.7 (2001 Jun 25): 1427-40.

    The Journal of biological chemistry
    Gu J
    The Journal of biological chemistry 281.44 (2006 Nov 3): 33258-67.

    Experimental cell research
    Parrilla R
    Experimental cell research 314.10 (2008 Jun 10): 2004-15.

    Experimental cell research
    Kuentzel SL
    Experimental cell research 184.2 (1989 Oct): 407-26.

    Experimental cell research
    Juliano RL
    Experimental cell research 177.2 (1988 Aug): 303-18.

    The Journal of biological chemistry
    Gu J
    The Journal of biological chemistry 290.49 (2015 Dec 4): 29345-60.

    mAbs
    Soll DR
    mAbs 11.4 (2019 May/Jun): 691-708.

    FEBS letters
    Wewer UM
    FEBS letters 579.25 (2005 Oct 24): 5589-95.

    Journal of cell science
    Woods A
    Journal of cell science 113 ( Pt 3). (2000 Feb): 493-506.

    The Journal of cell biology
    Languino LR
    The Journal of cell biology 166.3 (2004 Aug 2): 407-18.

    Blood
    Miyata T
    Blood 113.21 (2009 May 21): 5304-13.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review