7E2

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$40.00
SKU: 7E2
View product citations for antibody 7E2 on CiteAb

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Available: 180

DSHB Data Sheet

Catalog Fields

Antigen: integrin beta-1 /CD29
Hybridoma Cells Available: Yes
Antigen Species: Chinese hamster
Depositor: Juliano, R.L.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: UNC - Chapel Hill
Positive Tested Species Reactivity: Chinese Hamster
Depositors Notes: This antibody recognizes a confirmational epitope on the beta subunit that is dependent upon disulfide bonding. 7E2 fails to recognize B1 integrin by immunoblot on WISH, HDF, BHK, NRK and NIH 3T3 cells.
Antigen Molecular Weight: 110 kDa (native)
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: I79_016350
Immunogen: Purified fibronectin receptor from CHO cells
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P2x63.Ag8.63
Epitope Location or Sequence:
Uniprot ID: G3HZ55 
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_532374 
Additional Information: RRID:AB_532374
Recommended Applications: FACS, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7E2 was deposited to the DSHB by Juliano, R.L. (DSHB Hybridoma Product 7E2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • FACS References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    ELISA References
    FACS References

    ADAM12-mediated focal adhesion formation is differently regulated by beta1 and beta3 integrins.
    Wewer UM
    FEBS letters 579.25 (2005 Oct 24): 5589-95.

    Control of extracellular matrix assembly by syndecan-2 proteoglycan.
    Woods A
    Journal of cell science 113 ( Pt 3). (2000 Feb): 493-506.

    Selective modulation of type 1 insulin-like growth factor receptor signaling and functions by beta1 integrins.
    Languino LR
    The Journal of cell biology 166.3 (2004 Aug 2): 407-18.

    Integrin-linked kinase associated with integrin activation.
    Miyata T
    Blood 113.21 (2009 May 21): 5304-13.

    All References

    Identification of CD9 extracellular domains important in regulation of CHO cell adhesion to fibronectin and fibronectin pericellular matrix assembly.
    Jennings LK
    Blood 100.13 (2002 Dec 15): 4502-11.

    Differential dynamics of alpha 5 integrin, paxillin, and alpha-actinin during formation and disassembly of adhesions in migrating cells.
    Horwitz AF
    The Journal of cell biology 153.7 (2001 Jun 25): 1427-40.

    N-glycosylation of the beta-propeller domain of the integrin alpha5 subunit is essential for alpha5beta1 heterodimerization, expression on the cell surface, and its biological function.
    Gu J
    The Journal of biological chemistry 281.44 (2006 Nov 3): 33258-67.

    Expression of podocalyxin enhances the adherence, migration, and intercellular communication of cells.
    Parrilla R
    Experimental cell research 314.10 (2008 Jun 10): 2004-15.

    Kinetic and morphological evidence for endocytosis of mammalian cell integrin receptors by using an anti-fibronectin receptor beta subunit monoclonal antibody.
    Kuentzel SL
    Experimental cell research 184.2 (1989 Oct): 407-26.

    Monoclonal antibodies to distinctive epitopes on the alpha and beta subunits of the fibronectin receptor.
    Juliano RL
    Experimental cell research 177.2 (1988 Aug): 303-18.

    Integrin α5 Suppresses the Phosphorylation of Epidermal Growth Factor Receptor and Its Cellular Signaling of Cell Proliferation via N-Glycosylation.
    Gu J
    The Journal of biological chemistry 290.49 (2015 Dec 4): 29345-60.

    ADAM12-mediated focal adhesion formation is differently regulated by beta1 and beta3 integrins.
    Wewer UM
    FEBS letters 579.25 (2005 Oct 24): 5589-95.

    Control of extracellular matrix assembly by syndecan-2 proteoglycan.
    Woods A
    Journal of cell science 113 ( Pt 3). (2000 Feb): 493-506.

    Selective modulation of type 1 insulin-like growth factor receptor signaling and functions by beta1 integrins.
    Languino LR
    The Journal of cell biology 166.3 (2004 Aug 2): 407-18.

    Integrin-linked kinase associated with integrin activation.
    Miyata T
    Blood 113.21 (2009 May 21): 5304-13.

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