7E2
Catalog Fields
Product Name/ID: 7E2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: I79_016350
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Clonality: Monoclonal
Immunogen: Purified fibronectin receptor from CHO cells
Clone:
Immunogen Sequence:
Myeloma Strain: P2x63.Ag8.63
Epitope Mapped: No
Antigen Name: integrin beta-1 /CD29
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 6/8/1994
Antigen Molecular Weight: 110 kDa (native)
Depositor: Juliano, R.L.
Antigen Sequence:
Depositor Institution: UNC - Chapel Hill
Antigen Species: Chinese hamster
Depositor Notes: This antibody recognizes a confirmational epitope on the beta subunit that is dependent upon disulfide bonding. 7E2 fails to recognize B1 integrin by immunoblot on WISH, HDF, BHK, NRK and NIH 3T3 cells.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chinese Hamster
Additional Information: RRID:AB_532374
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7E2 was deposited to the DSHB by Juliano, R.L. (DSHB Hybridoma Product 7E2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
12 References
Initial Publication
Monoclonal antibodies to distinctive epitopes on the alpha and beta subunits of the fibronectin receptor.
Juliano RL
Experimental cell research 177.2 (1988 Aug): 303-18.