Catalog Fields
Antigen: fibrinogen
Available to For-Profits: Yes
Antigen Species: mouse
Hybridoma Cells Available (Non-Profit): No
Isotype: Armenian Hamster IgG
Depositor: Coller, B.S.
Host Species: Armenian hamster
Antigen Sequence:
Positive Tested Species Reactivity: Human, Mouse
Depositors Institution: Rockefeller University
Antigen Molecular Weight:
Depositors Notes: Mouse and human fibrinogen. Function inhibition: inhibits fibrinogen to platelets; inhibits fibrinogen cross-linking by factor XIII; inhibits platelet-mediated clot retraction
Immunogen: mouse platelets
Gene:
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Antibody Registry ID: AB_2617393
Recommended Applications: ELISA, FACS, Immunoprecipitation
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7E9 was deposited to the DSHB by Coller, B.S. (DSHB Hybridoma Product 7E9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
1 Reference
Initial Publication
Preparation of monoclonal antibodies to murine platelet glycoprotein IIb/IIIa (alphaIIbbeta3) and other proteins from hamster-mouse interspecies hybridomas.
Coller BS
Biochemical and biophysical research communications 262.1 (1999 Aug 19): 167-73.