7F4 (reovirus)
Catalog Fields
Clone ID/Product Name: 7F4 (reovirus)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: S4
Ab Isotype: MIgG2b, kappa light chain
Gene Name:
Antibody Registry ID: AB_2617411
Uniprot ID: P07939
Entrez Gene ID: 8437031
Clonality: Monoclonal
Immunogen: T3D intact virus
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: P3 Ag8.6.5.3
Epitope Mapped: No
Antigen Name: reovirus sigma 3
Epitope Location or Sequence: Epitope resides within sigma 3
Alternate Antigen Name:
Deposit Date: 8/6/2013
Antigen Molecular Weight: 41 kDa
Depositor: Dermody, T.S.
Antigen Sequence:
Depositor Institution: University of Pittsburgh School of Medicine
Antigen Species: Mammalian orthoreovirus strain Type 3 Dearing (T3D)
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Type 1 Lang (T1L), Type 3 Dearing (T3D)
Additional Information: RRID:AB_2617411
Recommended Applications: ELISA, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7F4 (reovirus) was deposited to the DSHB by Dermody, T.S. (DSHB Hybridoma Product 7F4 (reovirus))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
1 Reference
Initial Publication
Monoclonal antibodies to reovirus reveal structure/function relationships between capsid proteins and genetics of susceptibility to antibody action.
Tyler KL
Journal of virology 65.12 (1991 Dec): 6772-81.