8-1E7

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$40.00
SKU: 8-1E7
View product citations for antibody 8-1E7 on CiteAb

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Available: 116

DSHB Data Sheet

Catalog Fields

Antigen: Glial fibrillary acidic protein (GFAP)
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: De Blas, Angel L.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Connecticut
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes: This antibody It labels astrocytes, Bergmann glia fibers in cerebellum and Muller cells in retina.
Antigen Molecular Weight: 49.9 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Gfap
Immunogen: Synaptosomal plasma membrane fraction from cerebral cortex
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Location or Sequence:
Uniprot ID: P47819 
Immunogen Sequence: Full length protein
Entrez Gene ID: 24387 
Additional Characterization:  
Antibody Registry ID: AB_2753228 
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8-1E7 was deposited to the DSHB by De Blas, Angel L. (DSHB Hybridoma Product 8-1E7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • All References
  • Initial Publication

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    IF References
    WB References

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    IHC References
    IP References

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    ELISA References

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    All References

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