8-6A2

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$45.00
SKU: 8-6A2
View product citations for antibody 8-6A2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Microtubule-associated protein 2 (MAP-2)
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: De Blas, Angel L.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Connecticut
Positive Tested Species Reactivity: Rat
Depositors Notes: This antibody labels Purkinje cells and other large neurons.
Antigen Molecular Weight: 202.4 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Map2
Immunogen: Synaptosomal plasma membrane fraction from cerebral cortex
Alternate Gene Names: MAP2R, Mtap2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Location or Sequence:
Uniprot ID: P15146 
Immunogen Sequence: Full length protein
Entrez Gene ID: 25595 
Additional Characterization:  
Antibody Registry ID: AB_2877073 
Additional Information:
Recommended Applications: ELISA, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8-6A2 was deposited to the DSHB by De Blas, Angel L. (DSHB Hybridoma Product 8-6A2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IHC References
  • All References
  • Initial Publication

    Monoclonal antibodies to specific astroglial and neuronal antigens reveal the cytoarchitecture of the Bergmann glia fibers in the cerebellum.
    de Blas AL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 4.1 (1984 Jan): 265-73.

    IHC References

    Localization of the GABAA receptor in the rat brain with a monoclonal antibody to the 57,000 Mr peptide of the GABAA receptor/benzodiazepine receptor/Cl- channel complex.
    Friedrich P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 602-14.

    Monoclonal antibodies to specific astroglial and neuronal antigens reveal the cytoarchitecture of the Bergmann glia fibers in the cerebellum.
    de Blas AL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 4.1 (1984 Jan): 265-73.

    All References

    Localization of the GABAA receptor in the rat brain with a monoclonal antibody to the 57,000 Mr peptide of the GABAA receptor/benzodiazepine receptor/Cl- channel complex.
    Friedrich P
    The Journal of neuroscience : the official journal of the Society for Neuroscience 8.2 (1988 Feb): 602-14.

    Monoclonal antibodies to specific astroglial and neuronal antigens reveal the cytoarchitecture of the Bergmann glia fibers in the cerebellum.
    de Blas AL
    The Journal of neuroscience : the official journal of the Society for Neuroscience 4.1 (1984 Jan): 265-73.

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