8-7A5

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$45.00 to $425.00
SKU: 8-7A5
View product citations for antibody 8-7A5 on CiteAb

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Available: 39

DSHB Data Sheet

Catalog Fields

Antigen: actin
Available to For-Profits: Yes
Antigen Species: Rat
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: De Blas, Angel L.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Buffalo, Rat
Depositors Institution: University of Connecticut
Antigen Molecular Weight: 41.7 kDa
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Synaptosomal plasma membrane fraction from cerebral cortex
Gene: Actb
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Mapped: No
Uniprot ID: P60711 
Epitope Location or Sequence:
Entrez Gene ID: 81822 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2722791 
Additional Characterization:  
Recommended Applications: ELISA, Immunohistochemistry, Western Blot
Additional Information: RRID: AB_2722791
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8-7A5 was deposited to the DSHB by De Blas, Angel L. (DSHB Hybridoma Product 8-7A5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • All References
  • Initial Publication

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    WB References

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    Elucidating the processes and pathways enriched in buffalo sperm proteome in regulating semen quality.
    Bhatta R
    Cell and tissue research 383.2 (2021 Feb): 881-903.

    IHC References

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    IP References

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    ELISA References

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    All References

    Mammalian brain antigens defined by monoclonal antibodies.
    Cherwinski HM
    Brain research 322.2 (1984 Nov 26): 277-87.

    Elucidating the processes and pathways enriched in buffalo sperm proteome in regulating semen quality.
    Bhatta R
    Cell and tissue research 383.2 (2021 Feb): 881-903.

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