8A2

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$40.00
SKU: 8A2
View product citations for antibody 8A2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: neural associated ganglioside (9-0-acetyl-ganglioside)
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Lemmon, V.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Miami School of Medicine
Positive Tested Species Reactivity: Chicken, Fish, Human, Mammal, Rat
Depositors Notes: 8A2 binds to neurons in all vertebrate species tested, from fish to mammals. 8A2 gangliosides are a set of rare gangliosides with a base labile epitope, suggesting the presence of a 9-0-acetyl group. The 8A2 gangliosides are different from the JONES and D1.1 gangliosides. 8A2 causes growth cone collapse of goldfish retinal ganglion cell axons. 8A2 gives an intense staining of chick embryo axons in vivo and in vitro by binding to the surface of neurons, predominantly on their axons. Tissues fixed with 2% paraformaldehyde and 0.01 glutaraldehyde, work well for immunohistology.
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: Chicken optic nerve (E14)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_531867 
Additional Information: Gangliosides are glycosphingolipids with one or more sialic acids. Western blot references are for TLC immunooverlay.
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8A2 was deposited to the DSHB by Lemmon, V. (DSHB Hybridoma Product 8A2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FB References
  • All References
  • Initial Publication

    Developmental biology
    Lemmon V
    Developmental biology 145.1 (1991 May): 154-63.

    IF References

    Developmental biology
    Koenig E
    Developmental biology 156.1 (1993 Mar): 230-42.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Sanes JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.6 (1995 Jun): 4556-71.

    WB References

    Developmental biology
    Lemmon V
    Developmental biology 145.1 (1991 May): 154-63.

    The Journal of biological chemistry
    Varki A
    The Journal of biological chemistry 267.23 (1992 Aug 15): 16200-11.

    IHC References

    Developmental biology
    Lemmon V
    Developmental biology 145.1 (1991 May): 154-63.

    The Anatomical record
    Black AC Jr
    The Anatomical record 263.4 (2001 Aug 1): 350-60.

    Current topics in microbiology and immunology
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    ELISA References

    Developmental biology
    Lemmon V
    Developmental biology 145.1 (1991 May): 154-63.

    FB References

    Developmental biology
    Koenig E
    Developmental biology 156.1 (1993 Mar): 230-42.

    All References

    Developmental biology
    Lemmon V
    Developmental biology 145.1 (1991 May): 154-63.

    The Anatomical record
    Black AC Jr
    The Anatomical record 263.4 (2001 Aug 1): 350-60.

    Current topics in microbiology and immunology
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Developmental biology
    Koenig E
    Developmental biology 156.1 (1993 Mar): 230-42.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Sanes JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.6 (1995 Jun): 4556-71.

    The Journal of biological chemistry
    Varki A
    The Journal of biological chemistry 267.23 (1992 Aug 15): 16200-11.

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