8A2
Catalog Fields
Clone ID/Product Name: 8A2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgM
Gene Name:
Antibody Registry ID: AB_531867
Clonality: Monoclonal
Immunogen: Chicken optic nerve (E14)
Clone:
Immunogen Sequence:
Myeloma Strain: NS1
Epitope Mapped: No
Antigen Name: neural associated ganglioside (9-0-acetyl-ganglioside)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/20/1991
Antigen Molecular Weight:
Depositor: Lemmon, V.
Antigen Sequence:
Depositor Institution: University of Miami School of Medicine
Antigen Species: Chicken
Depositor Notes: 8A2 binds to neurons in all vertebrate species tested, from fish to mammals. 8A2 gangliosides are a set of rare gangliosides with a base labile epitope, suggesting the presence of a 9-0-acetyl group. The 8A2 gangliosides are different from the JONES and D1.1 gangliosides. 8A2 causes growth cone collapse of goldfish retinal ganglion cell axons. 8A2 gives an intense staining of chick embryo axons in vivo and in vitro by binding to the surface of neurons, predominantly on their axons. Tissues fixed with 2% paraformaldehyde and 0.01 glutaraldehyde, work well for immunohistology.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Fish, Human, Mammal, Rat
Additional Information: Gangliosides are glycosphingolipids with one or more sialic acids. Western blot references are for TLC immunooverlay.
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8A2 was deposited to the DSHB by Lemmon, V. (DSHB Hybridoma Product 8A2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
8 References
Initial Publication
Studies of the developing chick retina using monoclonal antibody 8A2 that recognizes a novel set of gangliosides.
Lemmon V
Developmental biology 145.1 (1991 May): 154-63.