8A2
Catalog Fields
Clone ID/Product Name: 8A2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgM
Gene Name:
Antibody Registry ID: AB_531867
Clonality: Monoclonal
Immunogen: Chicken optic nerve (E14)
Clone:
Immunogen Sequence:
Myeloma Strain: NS1
Epitope Mapped: No
Antigen Name: neural associated ganglioside (9-0-acetyl-ganglioside)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/20/1991
Antigen Molecular Weight:
Depositor: Lemmon, V.
Antigen Sequence:
Depositor Institution: University of Miami School of Medicine
Antigen Species: Chicken
Depositor Notes: 8A2 binds to neurons in all vertebrate species tested, from fish to mammals. 8A2 gangliosides are a set of rare gangliosides with a base labile epitope, suggesting the presence of a 9-0-acetyl group. The 8A2 gangliosides are different from the JONES and D1.1 gangliosides. 8A2 causes growth cone collapse of goldfish retinal ganglion cell axons. 8A2 gives an intense staining of chick embryo axons in vivo and in vitro by binding to the surface of neurons, predominantly on their axons. Tissues fixed with 2% paraformaldehyde and 0.01 glutaraldehyde, work well for immunohistology.
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Fish, Human, Mammal, Rat
Additional Information: Gangliosides are glycosphingolipids with one or more sialic acids. Western blot references are for TLC immunooverlay.
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot