8A2

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$40.00
SKU: 8A2
View product citations for antibody 8A2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: neural associated ganglioside (9-0-acetyl-ganglioside)
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Lemmon, V.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Miami School of Medicine
Positive Tested Species Reactivity: Chicken, Fish, Human, Mammal, Rat
Depositors Notes: 8A2 binds to neurons in all vertebrate species tested, from fish to mammals. 8A2 gangliosides are a set of rare gangliosides with a base labile epitope, suggesting the presence of a 9-0-acetyl group. The 8A2 gangliosides are different from the JONES and D1.1 gangliosides. 8A2 causes growth cone collapse of goldfish retinal ganglion cell axons. 8A2 gives an intense staining of chick embryo axons in vivo and in vitro by binding to the surface of neurons, predominantly on their axons. Tissues fixed with 2% paraformaldehyde and 0.01 glutaraldehyde, work well for immunohistology.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Chicken optic nerve (E14)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_531867 
Additional Information: Gangliosides are glycosphingolipids with one or more sialic acids. Western blot references are for TLC immunooverlay.
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8A2 was deposited to the DSHB by Lemmon, V. (DSHB Hybridoma Product 8A2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FB References
  • All References
  • Initial Publication
    IF References

    Monoclonal antibody 8A2-induced retraction appears to be mediated by protein phosphorylation in goldfish retinal ganglion cell axons.
    Koenig E
    Developmental biology 156.1 (1993 Mar): 230-42.

    Lamina-specific expression of adhesion molecules in developing chick optic tectum.
    Sanes JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.6 (1995 Jun): 4556-71.

    WB References
    IHC References

    Studies of the developing chick retina using monoclonal antibody 8A2 that recognizes a novel set of gangliosides.
    Lemmon V
    Developmental biology 145.1 (1991 May): 154-63.

    Clonogenic analysis reveals reserve stem cells in postnatal mammals: I. Pluripotent mesenchymal stem cells.
    Black AC Jr
    The Anatomical record 263.4 (2001 Aug 1): 350-60.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    ELISA References
    FB References
    All References

    Studies of the developing chick retina using monoclonal antibody 8A2 that recognizes a novel set of gangliosides.
    Lemmon V
    Developmental biology 145.1 (1991 May): 154-63.

    Clonogenic analysis reveals reserve stem cells in postnatal mammals: I. Pluripotent mesenchymal stem cells.
    Black AC Jr
    The Anatomical record 263.4 (2001 Aug 1): 350-60.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Monoclonal antibody 8A2-induced retraction appears to be mediated by protein phosphorylation in goldfish retinal ganglion cell axons.
    Koenig E
    Developmental biology 156.1 (1993 Mar): 230-42.

    Lamina-specific expression of adhesion molecules in developing chick optic tectum.
    Sanes JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 15.6 (1995 Jun): 4556-71.

    Structural and immunological characterization of O-acetylated GD2. Evidence that GD2 is an acceptor for ganglioside O-acetyltransferase in human melanoma cells.
    Varki A
    The Journal of biological chemistry 267.23 (1992 Aug 15): 16200-11.

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