8D9

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$40.00
SKU: 8D9
View product citations for antibody 8D9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: L1-like CAM (avian)
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Lemmon, V.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: The Miami Project to Cure Paralysis, University of Miami Sch. of Med.
Positive Tested Species Reactivity: Chick
Depositors Notes: Specificity note: Binds to surface of chick neurons, predominantly on their axons. Binds to chick but not to quail or mammals. Immunohistology note: stains tissue fixed with 2% paraformaldehyde and 0.01 glutaraldehyde, does not stain paraffin embedded tissue. IP note: antibody does not work on Western blots but is very useful for immunoprecipitations and affinity chromatography. Functional effects: polyclonal antibodies to 8D9 antigen inhibit axon fasciculation, 8D9 antigen is a potent substrate for neurite growth. IHC note: monoclonal antibody 8D9 gives intense staining of chick axons in vivo and in vitro.
Antigen Molecular Weight: 190 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: chick optic nerve
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8D9 was deposited to the DSHB by Lemmon, V. (DSHB Hybridoma Product 8D9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    The appearance of an L1-like molecule in the chick primary visual pathway.
    McLoon SC
    The Journal of neuroscience : the official journal of the Society for Neuroscience 6.10 (1986 Oct): 2987-94.

    All References

    The appearance of an L1-like molecule in the chick primary visual pathway.
    McLoon SC
    The Journal of neuroscience : the official journal of the Society for Neuroscience 6.10 (1986 Oct): 2987-94.

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