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SKU: 8D9

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 8D9
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_531781 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: chick optic nerve
Immunogen Sequence:
Myeloma Strain: NS1
Epitope Mapped: No
Antigen Name: L1-like CAM (avian)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/20/1991
Antigen Molecular Weight: 190 kDa
Depositor: Lemmon, V.
Antigen Sequence:
Depositor Institution: Miami Project to Cure Paralysis, University of Miami Sch. of Med.
Antigen Species: chicken
Depositor Notes: Specificity note: Binds to surface of chick neurons, predominantly on their axons. Binds to chick but not to quail or mammals. Immunohistology note: stains tissue fixed with 2% paraformaldehyde and 0.01 glutaraldehyde, does not stain paraffin embedded tissue. IP note: antibody does not work on Western blots but is very useful for immunoprecipitations and affinity chromatography. Functional effects: polyclonal antibodies to 8D9 antigen inhibit axon fasciculation, 8D9 antigen is a potent substrate for neurite growth. IHC note: monoclonal antibody 8D9 gives intense staining of chick axons in vivo and in vitro.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chick
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8D9 was deposited to the DSHB by Lemmon, V. (DSHB Hybridoma Product 8D9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    The appearance of an L1-like molecule in the chick primary visual pathway.
    McLoon SC
    The Journal of neuroscience : the official journal of the Society for Neuroscience 6.10 (1986 Oct): 2987-94.

    All References

    The appearance of an L1-like molecule in the chick primary visual pathway.
    McLoon SC
    The Journal of neuroscience : the official journal of the Society for Neuroscience 6.10 (1986 Oct): 2987-94.

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