8F8 (3C5)

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$45.00 to $425.00
SKU: 8F8 (3C5)
View product citations for antibody 8F8 (3C5) on CiteAb

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 8F8 (3C5)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Aoah
Ab Isotype: MIgG2a
Gene Symbol:
Antibody Registry ID: AB_2877054 
Uniprot ID: P28039 
Entrez Gene ID: 313 
Clonality: Monoclonal
Immunogen: Expression plasmid & Recombinant human AOAH
Immunogen Sequence: Full length sequence
Myeloma Strain: SP2/0-IL-6
Epitope Mapped: No
Antigen Name: Acyloxyacyl hydrolase (AOAH)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 10/21/2020
Antigen Molecular Weight: 65.1 kDa
Depositor: Munford, R.S.
Antigen Sequence:
Depositor Institution: NIAID, NIH, Clinical Immunology and Microbiology
Antigen Species: Human
Depositor Notes: This antibody recognizes the large subunit of mature Aoah. Aoah is a lipase important in the removal of fatty acyl chains from bacterial LPS.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse
Additional Information: RRID:AB_2877054
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8F8 (3C5) was deposited to the DSHB by Munford, R.S. (DSHB Hybridoma Product 8F8 (3C5))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

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