8H9
Catalog Fields
Antigen: Fc-gamma receptor I/CD64
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Hildreth, J.E.K.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: Johns Hopkins University School of Medicine
Antigen Molecular Weight: 42.6 kDa
Depositors Notes: This antigen is also known as the high affinity immunoglobulin gamma FC receptor I.
Immunogen:
Gene: FCGR1A
Alternate Antibody Name:
Alternate Gene Names: CD64A, FCG1, FCGR1, IGFR1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X653-Ag8
Epitope Mapped: No
Uniprot ID: P12314
Epitope Location or Sequence:
Entrez Gene ID: 2209
Immunogen Sequence:
Antibody Registry ID: AB_528159
Recommended Applications: Function Blocking
Additional Information: RRID: AB_528159
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8H9 was deposited to the DSHB by Hildreth, J.E.K. (DSHB Hybridoma Product 8H9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
1 Reference