Antigen: Fc-gamma receptor I/CD64
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Hildreth, J.E.K.
Host Species: mouse
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Human
Depositors Notes: This antigen is also known as the high affinity immunoglobulin gamma FC receptor I.
Antigen Molecular Weight: 42.6 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Alternate Gene Names: CD64A, FCG1, FCGR1, IGFR1
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: P3X653-Ag8
Epitope Location or Sequence:
Uniprot ID: P12314
Entrez Gene ID: 2209
Antibody Registry ID: AB_528159
Additional Information: RRID: AB_528159
Recommended Applications: Function Blocking
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8H9 was deposited to the DSHB by Hildreth, J.E.K. (DSHB Hybridoma Product 8H9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).