8H9

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$40.00
SKU: 8H9
View product citations for antibody 8H9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Fc-gamma receptor I/CD64
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Hildreth, J.E.K.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Human
Depositors Notes: This antigen is also known as the high affinity immunoglobulin gamma FC receptor I.
Antigen Molecular Weight: 42.6 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: FCGR1A
Immunogen:
Alternate Gene Names: CD64A, FCG1, FCGR1, IGFR1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X653-Ag8
Epitope Location or Sequence:
Uniprot ID: P12314 
Immunogen Sequence:
Entrez Gene ID: 2209 
Additional Characterization:  
Antibody Registry ID: AB_528159 
Additional Information: RRID: AB_528159
Recommended Applications: Function Blocking
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8H9 was deposited to the DSHB by Hildreth, J.E.K. (DSHB Hybridoma Product 8H9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • FB References
  • All References
  • FB References

    Fc gamma receptor-mediated activation of phospholipase D regulates macrophage phagocytosis of IgG-opsonized particles.
    Jackson S
    Journal of immunology (Baltimore, Md. : 1950) 162.4 (1999 Feb 15): 2266-74.

    All References

    Fc gamma receptor-mediated activation of phospholipase D regulates macrophage phagocytosis of IgG-opsonized particles.
    Jackson S
    Journal of immunology (Baltimore, Md. : 1950) 162.4 (1999 Feb 15): 2266-74.

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