8H9

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$40.00
SKU: 8H9
View product citations for antibody 8H9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Fc-gamma receptor I/CD64
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Hildreth, J.E.K.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Human
Depositors Notes: This antigen is also known as the high affinity immunoglobulin gamma FC receptor I.
Antigen Molecular Weight: 42.6 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: FCGR1A
Immunogen:
Alternate Gene Names: CD64A, FCG1, FCGR1, IGFR1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3X653-Ag8
Epitope Location or Sequence:
Uniprot ID: P12314 
Immunogen Sequence:
Entrez Gene ID: 2209 
Additional Characterization:
Antibody Registry ID: AB_528159 
Additional Information: RRID: AB_528159
Recommended Applications: Function Blocking
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8H9 was deposited to the DSHB by Hildreth, J.E.K. (DSHB Hybridoma Product 8H9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • FB References
  • All References
  • FB References

    Fc gamma receptor-mediated activation of phospholipase D regulates macrophage phagocytosis of IgG-opsonized particles.
    Jackson S
    Journal of immunology (Baltimore, Md. : 1950) 162.4 (1999 Feb 15): 2266-74.

    All References

    Fc gamma receptor-mediated activation of phospholipase D regulates macrophage phagocytosis of IgG-opsonized particles.
    Jackson S
    Journal of immunology (Baltimore, Md. : 1950) 162.4 (1999 Feb 15): 2266-74.

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