9.1 ITGA7
Catalog Fields
Clone ID/Product Name: 9.1 ITGA7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: ITGA7
Ab Isotype: MIgG2c
Gene Name:
Antibody Registry ID: AB_2721955
Uniprot ID: Q13683
Entrez Gene ID: 3679
Clonality: Monoclonal
Immunogen: Live NIH3T3 cells transfected with human alpha 7 integrin DNA
Clone:
Immunogen Sequence:
Myeloma Strain: Sp2/0
Epitope Mapped: No
Antigen Name: Integrin alpha-7, extracellular domain
Epitope Location or Sequence: Extracellular domain of alpha 7 integrin
Alternate Antigen Name:
Deposit Date: 9/7/2016
Antigen Molecular Weight: 128 kDa
Depositor: Kramer, R.H.
Antigen Sequence:
Depositor Institution: University of California, San Francisco
Antigen Species: Human
Depositor Notes: The 9.1 antibody recognizes the extracellular domain of alpha 7 integrin in both native and denatured conformations.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: Alpha 7/beta-1 integrin is the primary laminin-1 receptor. This receptor is expressed in skeletal and cardiac muscle and certain tumor cells. RRID:AB_2721955
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9.1 ITGA7 was deposited to the DSHB by Kramer, R.H. (DSHB Hybridoma Product 9.1 ITGA7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
7 References
Initial Publication
Transfection of MCF-7 carcinoma cells with human integrin alpha7 cDNA promotes adhesion to laminin.
Kramer RH
Archives of biochemistry and biophysics 385.1 (2001 Jan 1): 108-16.