9.1 ITGA7

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$40.00
SKU: 9.1 ITGA7
View product citations for antibody 9.1 ITGA7 on CiteAb

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Available: 117

DSHB Data Sheet

Catalog Fields

Antigen: Integrin alpha-7, extracellular domain
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Kramer, R.H.
Isotype: MIgG2c
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California at San Francisco
Positive Tested Species Reactivity: Human
Depositors Notes: The 9.1 antibody recognizes the extracellular domain of alpha 7 integrin in both native and denatured conformations.
Antigen Molecular Weight: 128 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ITGA7
Immunogen: Live NIH3T3 cells transfected with human alpha 7 integrin DNA
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Sp2/0
Epitope Location or Sequence: Extracellular domain of alpha 7 integrin
Uniprot ID: Q13683 
Immunogen Sequence:
Entrez Gene ID: 3679 
Additional Characterization:
Antibody Registry ID: AB_2721955 
Additional Information: Alpha 7/beta-1 integrin is the primary laminin-1 receptor. This receptor is expressed in skeletal and cardiac muscle and certain tumor cells. RRID:AB_2721955
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9.1 ITGA7 was deposited to the DSHB by Kramer, R.H. (DSHB Hybridoma Product 9.1 ITGA7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FB References
  • FACS References
  • All References
  • Initial Publication

    Transfection of MCF-7 carcinoma cells with human integrin alpha7 cDNA promotes adhesion to laminin.
    Kramer RH
    Archives of biochemistry and biophysics 385.1 (2001 Jan 1): 108-16.

    IF References

    alpha7 integrin expressing human fetal myogenic progenitors have stem cell-like properties and are capable of osteogenic differentiation.
    Kramer RH
    Experimental cell research 312.20 (2006 Dec 10): 4162-80.

    Transfection of MCF-7 carcinoma cells with human integrin alpha7 cDNA promotes adhesion to laminin.
    Kramer RH
    Archives of biochemistry and biophysics 385.1 (2001 Jan 1): 108-16.

    Analysis of human muscle stem cells reveals a differentiation-resistant progenitor cell population expressing Pax7 capable of self-renewal.
    Kramer RH
    Developmental dynamics : an official publication of the American Association of Anatomists 238.1 (2009 Jan): 138-49.

    Laminin production and basement membrane deposition by mesenchymal stem cells upon adipogenic differentiation.
    Korhonen M
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 61.10 (2013 Oct): 719-30.

    WB References

    Transfection of MCF-7 carcinoma cells with human integrin alpha7 cDNA promotes adhesion to laminin.
    Kramer RH
    Archives of biochemistry and biophysics 385.1 (2001 Jan 1): 108-16.

    The beta1 cytoplasmic domain regulates the laminin-binding specificity of the alpha7X1 integrin.
    Kramer RH
    Molecular biology of the cell 14.9 (2003 Sep): 3507-18.

    IP References

    The beta1 cytoplasmic domain regulates the laminin-binding specificity of the alpha7X1 integrin.
    Kramer RH
    Molecular biology of the cell 14.9 (2003 Sep): 3507-18.

    Transfection of MCF-7 carcinoma cells with human integrin alpha7 cDNA promotes adhesion to laminin.
    Kramer RH
    Archives of biochemistry and biophysics 385.1 (2001 Jan 1): 108-16.

    FB References
    FACS References

    The beta1 cytoplasmic domain regulates the laminin-binding specificity of the alpha7X1 integrin.
    Kramer RH
    Molecular biology of the cell 14.9 (2003 Sep): 3507-18.

    Transfection of MCF-7 carcinoma cells with human integrin alpha7 cDNA promotes adhesion to laminin.
    Kramer RH
    Archives of biochemistry and biophysics 385.1 (2001 Jan 1): 108-16.

    alpha7 integrin expressing human fetal myogenic progenitors have stem cell-like properties and are capable of osteogenic differentiation.
    Kramer RH
    Experimental cell research 312.20 (2006 Dec 10): 4162-80.

    Laminin isoforms of lymph nodes and predominant role of alpha5-laminin(s) in adhesion and migration of blood lymphocytes.
    Patarroyo M
    Journal of leukocyte biology 84.3 (2008 Sep): 701-12.

    Analysis of human muscle stem cells reveals a differentiation-resistant progenitor cell population expressing Pax7 capable of self-renewal.
    Kramer RH
    Developmental dynamics : an official publication of the American Association of Anatomists 238.1 (2009 Jan): 138-49.

    Differentiation of human skeletal muscle stem cells into odontoblasts is dependent on induction of α1 integrin expression.
    Kramer RH
    The Journal of biological chemistry 289.20 (2014 May 16): 14380-91.

    All References

    alpha7 integrin expressing human fetal myogenic progenitors have stem cell-like properties and are capable of osteogenic differentiation.
    Kramer RH
    Experimental cell research 312.20 (2006 Dec 10): 4162-80.

    Transfection of MCF-7 carcinoma cells with human integrin alpha7 cDNA promotes adhesion to laminin.
    Kramer RH
    Archives of biochemistry and biophysics 385.1 (2001 Jan 1): 108-16.

    Analysis of human muscle stem cells reveals a differentiation-resistant progenitor cell population expressing Pax7 capable of self-renewal.
    Kramer RH
    Developmental dynamics : an official publication of the American Association of Anatomists 238.1 (2009 Jan): 138-49.

    Laminin production and basement membrane deposition by mesenchymal stem cells upon adipogenic differentiation.
    Korhonen M
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 61.10 (2013 Oct): 719-30.

    The beta1 cytoplasmic domain regulates the laminin-binding specificity of the alpha7X1 integrin.
    Kramer RH
    Molecular biology of the cell 14.9 (2003 Sep): 3507-18.

    Differentiation of human skeletal muscle stem cells into odontoblasts is dependent on induction of α1 integrin expression.
    Kramer RH
    The Journal of biological chemistry 289.20 (2014 May 16): 14380-91.

    Laminin isoforms of lymph nodes and predominant role of alpha5-laminin(s) in adhesion and migration of blood lymphocytes.
    Patarroyo M
    Journal of leukocyte biology 84.3 (2008 Sep): 701-12.

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