Antigen: integrin beta-3 (CD61), human
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Strom, D.
Host Species: mouse
Depositors Institution: Marian University
Positive Tested Species Reactivity: Human
Depositors Notes: 9H5 recognizes wild type integrin beta3 (HPA-1a) but not the L33P variant (HPA-1b).
Antigen Molecular Weight: Predicted; 87 kDa; Apparent: 90 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: peptide CSDEALPLGSPRCDL (aa 26-40 of integrin beta3)
Alternate Gene Names: GP3A, CD61
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Epitope Location or Sequence: aa 26-40 of integrin beta3
Uniprot ID: P05106
Immunogen Sequence: peptide
Entrez Gene ID: 3690
Antibody Registry ID: AB_2249330
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9H5 was deposited to the DSHB by Strom, D. (DSHB Hybridoma Product 9H5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).