A6 BCM

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$40.00
SKU: A6 BCM-s
View product citations for antibody A6 BCM on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: A6 Antigen in Biliary Epithelial & Oval cells
Hybridoma Cells Available:
Antigen Species: Mouse
Depositor: Factor, V.M.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: NIH
Positive Tested Species Reactivity: Mouse
Depositors Notes: This antibody is applicable on cultured cells, however the epitope is lost on primary cells after enzymatic digestion. In addition to biliary epithelium, A6 recognizes a wide variety of stratified and simple epithelium of ectodermal, mesodermal and endodermal origin in adult and embryonic tissues plus primary heptocarcinoma. See Table 3 in PMID 2292360.
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: DIPIN-treated OVAL cell lysate
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: X-63-Ag8-653
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_2618041 
Additional Information: This antibody recognizes the A6 antigen in frozen sections in unfixed, acetone, ethanol, methanol and paraformaldehyde fixed sections. Also, with antigen retrieval or enzymatic digestion with Proteinase K (20ug/ml in PBS;10-30 min), formalin-fixed paraffin embedded sections are sucessful labeled. RRID:AB_2618041
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
A6 BCM was deposited to the DSHB by Factor, V.M. (DSHB Hybridoma Product A6 BCM)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Common antigens of mouse oval and biliary epithelial cells. Expression on newly formed hepatocytes.
    Lasareva MN
    Differentiation; research in biological diversity 45.1 (1990 Oct): 29-37.

    IF References

    Hepatocyte growth factor/c-met signaling is required for stem-cell-mediated liver regeneration in mice.
    Thorgeirsson SS
    Hepatology (Baltimore, Md.) 55.4 (2012 Apr): 1215-26.

    Specific fate decisions in adult hepatic progenitor cells driven by MET and EGFR signaling.
    Thorgeirsson SS
    Genes and development 27.15 (2013 Aug 1): 1706-17.

    F-actin dynamics regulates mammalian organ growth and cell fate maintenance.
    Dupont S
    Journal of hepatology 71.1 (2019 Jul): 130-142.

    IHC References

    Common antigens of mouse oval and biliary epithelial cells. Expression on newly formed hepatocytes.
    Lasareva MN
    Differentiation; research in biological diversity 45.1 (1990 Oct): 29-37.

    Oval cells--hepatocytes relationships in Dipin-induced hepatocarcinogenesis in mice.
    Radaeva SA
    Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie 45.4 (1993 Aug): 239-44.

    Origin and fate of oval cells in dipin-induced hepatocarcinogenesis in the mouse.
    Thorgeirsson SS
    The American journal of pathology 145.2 (1994 Aug): 409-22.

    Hepatocyte growth factor/c-met signaling is required for stem-cell-mediated liver regeneration in mice.
    Thorgeirsson SS
    Hepatology (Baltimore, Md.) 55.4 (2012 Apr): 1215-26.

    Modeling pathogenesis of primary liver cancer in lineage-specific mouse cell types.
    Thorgeirsson SS
    Gastroenterology 145.1 (2013 Jul): 221-231.

    Specific fate decisions in adult hepatic progenitor cells driven by MET and EGFR signaling.
    Thorgeirsson SS
    Genes and development 27.15 (2013 Aug 1): 1706-17.

    All References

    Common antigens of mouse oval and biliary epithelial cells. Expression on newly formed hepatocytes.
    Lasareva MN
    Differentiation; research in biological diversity 45.1 (1990 Oct): 29-37.

    Oval cells--hepatocytes relationships in Dipin-induced hepatocarcinogenesis in mice.
    Radaeva SA
    Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie 45.4 (1993 Aug): 239-44.

    Origin and fate of oval cells in dipin-induced hepatocarcinogenesis in the mouse.
    Thorgeirsson SS
    The American journal of pathology 145.2 (1994 Aug): 409-22.

    Hepatocyte growth factor/c-met signaling is required for stem-cell-mediated liver regeneration in mice.
    Thorgeirsson SS
    Hepatology (Baltimore, Md.) 55.4 (2012 Apr): 1215-26.

    Modeling pathogenesis of primary liver cancer in lineage-specific mouse cell types.
    Thorgeirsson SS
    Gastroenterology 145.1 (2013 Jul): 221-231.

    Specific fate decisions in adult hepatic progenitor cells driven by MET and EGFR signaling.
    Thorgeirsson SS
    Genes and development 27.15 (2013 Aug 1): 1706-17.

    F-actin dynamics regulates mammalian organ growth and cell fate maintenance.
    Dupont S
    Journal of hepatology 71.1 (2019 Jul): 130-142.

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