A6 BCM
Catalog Fields
Clone ID/Product Name: A6 BCM
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: RIgG2a
Gene Name:
Antibody Registry ID: AB_2618041
Clonality: Monoclonal
Immunogen: DIPIN-treated OVAL cell lysate
Clone:
Immunogen Sequence:
Myeloma Strain: X-63-Ag8-653
Epitope Mapped: No
Antigen Name: A6 Antigen in Biliary Epithelial & Oval cells
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/4/2015
Antigen Molecular Weight:
Depositor: Factor, V.M.
Antigen Sequence:
Depositor Institution: NIH
Antigen Species: Mouse
Depositor Notes: This antibody is applicable on cultured cells, however the epitope is lost on primary cells after enzymatic digestion. In addition to biliary epithelium, A6 recognizes a wide variety of stratified and simple epithelium of ectodermal, mesodermal and endodermal origin in adult and embryonic tissues plus primary heptocarcinoma. See Table 3 in PMID 2292360.
Host Species: rat
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Mouse
Additional Information: This antibody recognizes the A6 antigen in frozen sections in unfixed, acetone, ethanol, methanol and paraformaldehyde fixed sections. Also, with antigen retrieval or enzymatic digestion with Proteinase K (20ug/ml in PBS;10-30 min), formalin-fixed paraffin embedded sections are sucessful labeled. RRID:AB_2618041
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
A6 BCM was deposited to the DSHB by Factor, V.M. (DSHB Hybridoma Product A6 BCM)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
7 References
Initial Publication
Common antigens of mouse oval and biliary epithelial cells. Expression on newly formed hepatocytes.
Lasareva MN
Differentiation; research in biological diversity 45.1 (1990 Oct): 29-37.