A6 BCM

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SKU: A6 BCM-s

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: A6 BCM
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: RIgG2a
Gene Name:
Antibody Registry ID: AB_2618041 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: DIPIN-treated OVAL cell lysate
Clone:
Immunogen Sequence:
Myeloma Strain: X-63-Ag8-653
Epitope Mapped: No
Antigen Name: A6 Antigen in Biliary Epithelial & Oval cells
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/4/2015
Antigen Molecular Weight:
Depositor: Factor, V.M.
Antigen Sequence:
Depositor Institution: NIH
Antigen Species: Mouse
Depositor Notes: This antibody is applicable on cultured cells, however the epitope is lost on primary cells after enzymatic digestion. In addition to biliary epithelium, A6 recognizes a wide variety of stratified and simple epithelium of ectodermal, mesodermal and endodermal origin in adult and embryonic tissues plus primary heptocarcinoma. See Table 3 in PMID 2292360.
Host Species: rat
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Mouse
Additional Information: This antibody recognizes the A6 antigen in frozen sections in unfixed, acetone, ethanol, methanol and paraformaldehyde fixed sections. Also, with antigen retrieval or enzymatic digestion with Proteinase K (20ug/ml in PBS;10-30 min), formalin-fixed paraffin embedded sections are sucessful labeled. RRID:AB_2618041
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
A6 BCM was deposited to the DSHB by Factor, V.M. (DSHB Hybridoma Product A6 BCM)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

7 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Common antigens of mouse oval and biliary epithelial cells. Expression on newly formed hepatocytes.
    Lasareva MN
    Differentiation; research in biological diversity 45.1 (1990 Oct): 29-37.

    IF References

    Hepatocyte growth factor/c-met signaling is required for stem-cell-mediated liver regeneration in mice.
    Thorgeirsson SS
    Hepatology (Baltimore, Md.) 55.4 (2012 Apr): 1215-26.

    Specific fate decisions in adult hepatic progenitor cells driven by MET and EGFR signaling.
    Thorgeirsson SS
    Genes & development 27.15 (2013 Aug 1): 1706-17.

    F-actin dynamics regulates mammalian organ growth and cell fate maintenance.
    Dupont S
    Journal of hepatology 71.1 (2019 Jul): 130-142.

    IHC References

    Common antigens of mouse oval and biliary epithelial cells. Expression on newly formed hepatocytes.
    Lasareva MN
    Differentiation; research in biological diversity 45.1 (1990 Oct): 29-37.

    Oval cells--hepatocytes relationships in Dipin-induced hepatocarcinogenesis in mice.
    Radaeva SA
    Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie 45.4 (1993 Aug): 239-44.

    Origin and fate of oval cells in dipin-induced hepatocarcinogenesis in the mouse.
    Thorgeirsson SS
    The American journal of pathology 145.2 (1994 Aug): 409-22.

    Hepatocyte growth factor/c-met signaling is required for stem-cell-mediated liver regeneration in mice.
    Thorgeirsson SS
    Hepatology (Baltimore, Md.) 55.4 (2012 Apr): 1215-26.

    Modeling pathogenesis of primary liver cancer in lineage-specific mouse cell types.
    Thorgeirsson SS
    Gastroenterology 145.1 (2013 Jul): 221-231.

    Specific fate decisions in adult hepatic progenitor cells driven by MET and EGFR signaling.
    Thorgeirsson SS
    Genes & development 27.15 (2013 Aug 1): 1706-17.

    All References

    Common antigens of mouse oval and biliary epithelial cells. Expression on newly formed hepatocytes.
    Lasareva MN
    Differentiation; research in biological diversity 45.1 (1990 Oct): 29-37.

    Oval cells--hepatocytes relationships in Dipin-induced hepatocarcinogenesis in mice.
    Radaeva SA
    Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie 45.4 (1993 Aug): 239-44.

    Origin and fate of oval cells in dipin-induced hepatocarcinogenesis in the mouse.
    Thorgeirsson SS
    The American journal of pathology 145.2 (1994 Aug): 409-22.

    Hepatocyte growth factor/c-met signaling is required for stem-cell-mediated liver regeneration in mice.
    Thorgeirsson SS
    Hepatology (Baltimore, Md.) 55.4 (2012 Apr): 1215-26.

    Modeling pathogenesis of primary liver cancer in lineage-specific mouse cell types.
    Thorgeirsson SS
    Gastroenterology 145.1 (2013 Jul): 221-231.

    Specific fate decisions in adult hepatic progenitor cells driven by MET and EGFR signaling.
    Thorgeirsson SS
    Genes & development 27.15 (2013 Aug 1): 1706-17.

    F-actin dynamics regulates mammalian organ growth and cell fate maintenance.
    Dupont S
    Journal of hepatology 71.1 (2019 Jul): 130-142.

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