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View product citations for antibody AF-CA2 on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: Aspergillus flavus isolate 93803
Hybridoma Cells Available: No
Antigen Species: Aspergillus flavus
Depositor: Dewey, F.M.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Oxford
Positive Tested Species Reactivity: All species of Aspergillus, All species of Penicillium
Depositors Notes: glycoprotein. Functional effects: The antibody has been used quantitatively, by plate and tube ELISA to determine the levels of infection of Aspergillus and Penicillium in grape berries. It has also been used to visualize fungi in swabs from the ears of patients suffering from chronic earache.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: PBS cell surface washings (5 ml) of a plate culture of Aspergillus flavus isolate 93803
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_10570544 
Additional Information:
Recommended Applications: ELISA
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AF-CA2 was deposited to the DSHB by Dewey, F.M. (DSHB Hybridoma Product AF-CA2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Otomycosis: the detection of fungi in ears by immunofluorescence microscopy.
    Gurr SJ
    Clinical otolaryngology and allied sciences 22.3 (1997 Jun): 275-83.

    All References

    Otomycosis: the detection of fungi in ears by immunofluorescence microscopy.
    Gurr SJ
    Clinical otolaryngology and allied sciences 22.3 (1997 Jun): 275-83.

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