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DSHB Data Sheet

Catalog Fields

Product Name/ID: AF-CA2
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgM
Gene Symbol:
Antibody Registry ID: AB_10570544 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: PBS cell surface washings (5 ml) of a plate culture of Aspergillus flavus isolate 93803
Immunogen Sequence:
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Antigen Name: Aspergillus flavus isolate 93803
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/9/2010
Antigen Molecular Weight:
Depositor: Dewey, F.M.
Antigen Sequence:
Depositor Institution: University of Oxford
Antigen Species: Aspergillus flavus
Depositor Notes: glycoprotein. Functional effects: The antibody has been used quantitatively, by plate and tube ELISA to determine the levels of infection of Aspergillus and Penicillium in grape berries. It has also been used to visualize fungi in swabs from the ears of patients suffering from chronic earache.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: All species of Aspergillus, All species of Penicillium
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AF-CA2 was deposited to the DSHB by Dewey, F.M. (DSHB Hybridoma Product AF-CA2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Otomycosis: the detection of fungi in ears by immunofluorescence microscopy.
    Gurr SJ
    Clinical otolaryngology and allied sciences 22.3 (1997 Jun): 275-83.

    All References

    Otomycosis: the detection of fungi in ears by immunofluorescence microscopy.
    Gurr SJ
    Clinical otolaryngology and allied sciences 22.3 (1997 Jun): 275-83.

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