Antigen: Aprataxin and PNKP like factor
Hybridoma Cells Available:
Antigen Species: Human
Depositor: EU Program Affinomics
Host Species: mouse
Depositors Institution: EMBL MACF
Positive Tested Species Reactivity: Human
Depositors Notes: This antigen is also known as apurinic-apyrimidinic endonuclease or XRCC1-interacting protein.
Antigen Molecular Weight: 56.9 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: E. coli expressed recombinant protein
Alternate Gene Names: C2orf13, MGC47799, Xip1, ZCCHH1
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: SP2/0-AG14
Epitope Location or Sequence:
Uniprot ID: Q8IW19
Immunogen Sequence: 19.7 kDa protein fragment
Entrez Gene ID: 200558
Antibody Registry ID: AB_2617446
Additional Information: RRID:AB_2617446
Recommended Applications: ELISA, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-APLF-15F11 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-APLF-15F11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).