Antigen: Rho guanine nucleotide exchange factor (GEF) 12
Depositor: EU Program Affinomics
Antigen Species: Human
Depositors Institution: EMBL MACF
Host Species: mouse
Alternate Gene Names: KIAA0382, LARG
Positive Tested Species Reactivity: Human
Antigen Molecular Weight: 173.2 kDa
Immunogen Sequence: 21.1 kDa protein fragment
Immunogen: E. coli expressed recombinant protein
Additional Information: This hybridoma was deposited as ARHGC-1-9H2 RRID:AB_2721959
Myeloma Strain: SP2/0-AG14
Uniprot ID: Q9NZN5
Entrez Gene ID: 23365
Antibody Registry ID: AB_2721959
Recommended Applications: ELISA, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-ARHGEF12-9H2 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-ARHGEF12-9H2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).