AFFN-BIRC3-10H11

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$40.00
SKU: AFFN-BIRC3-10H11-s
View product citations for antibody AFFN-BIRC3-10H11 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Baculoviral IAP repeat containing 3
Depositor: EU Program Affinomics
Antigen Species: Human
Depositors Institution: EMBL MACF
Isotype: MIgG1
Depositors Notes: This antigen is also known as apoptosis inhibitor 2 or TRAF signaling complex protein 1.
Host Species: mouse
Gene: BIRC3
Positive Tested Species Reactivity: Human
Alternate Gene Names: API2, IAP1, MIHC, RNF49
Antigen Molecular Weight: 68.3 kDa
Clonality: Monoclonal
Immunogen: E. coli expressed recombinant protein
Immunogen Sequence: 22.9 kDa protein fragment
Myeloma Strain: SP2/0-AG14
Additional Information: RRID:AB_2617458
Uniprot ID: Q13489 
Entrez Gene ID: 330 
Antibody Registry ID: AB_2617458 
Recommended Applications: ELISA, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-BIRC3-10H11 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-BIRC3-10H11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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