Antigen: Serine/threonine-protein kinase Chk2; Checkpoint kinase 2
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: EU Program Affinomics
Host Species: mouse
Depositors Institution: EMBL MACF
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody recognizes a serine/threonine protein kinase that is a required check-point to mediate cell cycle arrest, activation of DNA repair and apoptosis.
Predicted Species Reactivity:
Immunogen: E. coli expressed recombinant protein
Alternate Gene Names: bA444G7, CDS1, CHK2, HuCds1, PP1425, RAD53
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0-AG14
Epitope Location or Sequence:
Uniprot ID: O96017
Immunogen Sequence: a.a. 43-167
Entrez Gene ID: 11200
Additional Characterization: https://cordis.europa.eu/project/id/241481/reporting
Antibody Registry ID: AB_2721961
Additional Information: RRID:AB_2721961
Recommended Applications: ELISA, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-CHEK2-1H2 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-CHEK2-1H2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).