AFFN-KIF13B-7H5

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SKU: AFFN-KIF13B-7H5-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: AFFN-KIF13B-7H5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: KIF13B
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2721978 
Uniprot ID: Q9NQT8 
RRID:  
Entrez Gene ID: 23303 
Clonality: Monoclonal
Immunogen: E. coli expressed recombinant protein
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: SP2/0-AG14
Epitope Mapped: No
Antigen Name: Kinesin family member 13B
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 12/3/2014
Antigen Molecular Weight: 202.8 kDa
Depositor: EU Program Affinomics
Antigen Sequence:
Depositor Institution: EMBL MACF
Antigen Species: Human
Depositor Notes: This antigen is also known as kinesin-like protein GAKIN.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information: This hybridoma was deposited as KI13B-7H5 RRID:AB_2721978
Predicted Species Reactivity: Rabbit
Human Protein Atlas:  
Recommended Applications: ELISA, Immunofluorescence, Microarray
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-KIF13B-7H5 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-KIF13B-7H5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

3 References

  • Initial Publication
  • IF References
  • All References
  • Initial Publication
    IF References

    The RNA-Protein Interactome of Differentiated Kidney Tubular Epithelial Cells.
    Müller RU
    Journal of the American Society of Nephrology : JASN 30.4 (2019 Apr): 564-576.

    All References

    The RNA-Protein Interactome of Differentiated Kidney Tubular Epithelial Cells.
    Müller RU
    Journal of the American Society of Nephrology : JASN 30.4 (2019 Apr): 564-576.

    A roadmap to generate renewable protein binders to the human proteome.
    Gräslund S
    Nature methods 8.7 (2011 May 15): 551-8.

    ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome.
    Uhlén M
    Nature methods 4.1 (2007 Jan): 13-7.

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