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View product citations for antibody AFFN-PIK3R1-2E7 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Hybridoma Cells Available:
Antigen Species: Human
Depositor: EU Program Affinomics
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: EMBL MACF
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody recognizes a protein kinase subunit involved in insulin metabolism.
Antigen Molecular Weight: 83.5 kDa
Human Protein Atlas:
Predicted Species Reactivity: Mouse, Porcine, Rabbit, Rat
Gene: PIK3R1
Immunogen: Recombinant peptide
Alternate Gene Names: GRB1, p85, p85-ALPHA
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0-AG14
Epitope Location or Sequence:
Uniprot ID: P27986 
Immunogen Sequence: a.a.321-355
Entrez Gene ID: 5295 
Additional Characterization: Link 
Antibody Registry ID: AB_2617829 
Additional Information: This hybridoma was deposited as p85A-2E7. RRID:AB_2617829
Recommended Applications: ELISA, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-PIK3R1-2E7 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-PIK3R1-2E7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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