AFFN-PIK3R1-2E7

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$40.00
SKU: AFFN-PIK3R1-2E7-s
View product citations for antibody AFFN-PIK3R1-2E7 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: EU Program Affinomics
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: EMBL MACF
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody recognizes a protein kinase subunit involved in insulin metabolism.
Antigen Molecular Weight: 83.5 kDa
Human Protein Atlas:  
Predicted Species Reactivity: Mouse, Porcine, Rabbit, Rat
Gene: PIK3R1
Immunogen: Recombinant peptide
Alternate Gene Names: GRB1, p85, p85-ALPHA
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0-AG14
Epitope Location or Sequence:
Uniprot ID: P27986 
Immunogen Sequence: a.a.321-355
Entrez Gene ID: 5295 
Antibody Registry ID: AB_2617829 
Additional Information: This hybridoma was deposited as p85A-2E7. RRID:AB_2617829
Recommended Applications: ELISA, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-PIK3R1-2E7 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-PIK3R1-2E7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

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