AFFN-RNF8-3a10

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$40.00
SKU: AFFN-RNF8-3a10-s
View product citations for antibody AFFN-RNF8-3a10 on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Antigen: E3 ubiquitin-protein liase RNF8
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: EU Program Affinomics
Isotype: MIgG
Depositors Institution: EMBL MACF
Host Species: mouse
Depositors Notes: This antigen is also known as Ring finger protein 8, E3 ubiquitin protein ligase.
Positive Tested Species Reactivity: Human
Gene: rnf8
Antigen Molecular Weight: 55.5 kDa
Alternate Gene Names: KIAA0646
Immunogen: E. coli expressed recombinant protein
Clonality: Monoclonal
Myeloma Strain: SP2/0-AG14
Epitope Mapped: No
Uniprot ID: O76064 
Immunogen Sequence: 22.6 kDa protein fragment
Entrez Gene ID: 9025 
Additional Information: RRID:AB_2617871
Antibody Registry ID: AB_2617871 
Recommended Applications: ELISA, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-RNF8-3a10 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-RNF8-3a10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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