Catalog Fields
Antigen: Signal transducer and activator of transcription 1
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: EU Program Affinomics
Isotype: MIgG2c
Antigen Sequence:
Host Species: mouse
Depositors Institution: EMBL MACF
Positive Tested Species Reactivity: Human
Depositors Notes:
Predicted Species Reactivity: Mouse, Porcine, Rabbit, Rat
Gene: STAT1
Immunogen: E. coli expressed recombinant protein
Alternate Gene Names: 209750, CANDF7, ISGF-3, STAT91
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0-AG14
Epitope Location or Sequence:
Uniprot ID: P42224
Immunogen Sequence: a.a. 131-734; SH2 domain
Entrez Gene ID: 6772
Additional Characterization: https://cordis.europa.eu/project/id/241481/reporting
Antibody Registry ID: AB_2617926
Additional Information: RRID:AB_2617926
Recommended Applications: ELISA, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-STAT1-1B1 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-STAT1-1B1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
2 References
Initial Publication
A roadmap to generate renewable protein binders to the human proteome.
Gräslund S
Nature methods 8.7 (2011 May 15): 551-8.
ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome.
Uhlén M
Nature methods 4.1 (2007 Jan): 13-7.