AFFN-ZAP70-19H2

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$45.00
SKU: AFFN-ZAP70-19H2-s
View product citations for antibody AFFN-ZAP70-19H2 on CiteAb

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Available: 12

DSHB Data Sheet

Catalog Fields

Antigen: Tyrosine-protein kinase ZAP-70
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): No
Isotype: MIgG2c
Depositor: EU Program Affinomics
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: EMBL MACF
Antigen Molecular Weight: 69.7 kDa
Depositors Notes: This antibody recognizes a tyrosine kinase that is important in T-cell development and B-cell activation.
Predicted Species Reactivity: Mouse, Porcine, Rabbit, Rat
Human Protein Atlas:  
Immunogen: Recombinant peptide
Gene: ZAP70
Alternate Antibody Name:
Alternate Gene Names: SRK, STD, ZAP-70
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0-AG14
Epitope Mapped: No
Uniprot ID: P43403 
Epitope Location or Sequence:
Entrez Gene ID: 7535 
Immunogen Sequence: a.a. 1-268
Antibody Registry ID: AB_2618036 
Recommended Applications: ELISA, Microarray
Additional Information: RRID:AB_2618036
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-ZAP70-19H2 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-ZAP70-19H2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

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