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SKU: AFFN-ZAP70-19H2-s
View product citations for antibody AFFN-ZAP70-19H2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Tyrosine-protein kinase ZAP-70
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: EU Program Affinomics
Isotype: MIgG2a
Depositors Institution: EMBL MACF
Host Species: mouse
Depositors Notes: This antibody recognizes a tyrosine kinase that is important in T-cell development and B-cell activation.
Positive Tested Species Reactivity: Human
Gene: ZAP70
Antigen Molecular Weight: 69.7 kDa
Alternate Gene Names: SRK, STD, ZAP-70
Predicted Species Reactivity: Mouse, Porcine, Rabbit, Rat
Clonality: Monoclonal
Immunogen: Recombinant peptide
Epitope Mapped: No
Myeloma Strain: SP2/0-AG14
Immunogen Sequence: a.a. 1-268
Uniprot ID: P43403 
Entrez Gene ID: 7535 
Additional Information: RRID:AB_2618036
Antibody Registry ID: AB_2618036 
Recommended Applications: ELISA, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-ZAP70-19H2 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-ZAP70-19H2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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