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DSHB Data Sheet

Catalog Fields

Product Name/ID: AH6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_579783 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: nucleolus-like particles (NLP)
Immunogen Sequence:
Myeloma Strain: P3-X63Ag8
Epitope Mapped: No
Antigen Name: nucleolar protein
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 12/20/2005
Antigen Molecular Weight:
Depositor: Walsh, C.
Antigen Sequence:
Depositor Institution: University of Pittsburgh
Antigen Species: Naegleria gruberi
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: C. elegans, Mouse, Naegleria gruberi, Saccharomyces cerevisiae
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AH6 was deposited to the DSHB by Walsh, C. (DSHB Hybridoma Product AH6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Nucleolus-like morphology produced during the in vitro reassociation of nucleolar components.
    Walsh CJ
    The Journal of cell biology 122.4 (1993 Aug): 753-66.

    All References

    Nucleolus-like morphology produced during the in vitro reassociation of nucleolar components.
    Walsh CJ
    The Journal of cell biology 122.4 (1993 Aug): 753-66.

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