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View product citations for antibody ANTI-GRL(1) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: leukocyte secretory granules, chicken
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Thomas, J.-L.
Isotype: MIgG3
Depositors Institution: INSERM U495, Hopital de la Salpetriere
Host Species: mouse
Depositors Notes: Recognizes granulocytes, activated thrombocytes, acinar cells of the pancreas, and is a marker of secretory granules expressed on the plasma membrane of activated cells.
Positive Tested Species Reactivity: Chicken
Clonality: Monoclonal
Antigen Molecular Weight: Apparent: 92 kDa
Epitope Mapped: Yes
Immunogen: E15 bursa of Fabricius
Epitope Location or Sequence: Cell surface epitope
Alternate Antibody Name: I-A5
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0
Additional Information: Stains intracytoplasmic granules of granulocytes and thrombocytes and are present on the surface of these cells as a result of exocytosis of the intracytoplasmic granules. GRL1 is a protein shed or dsecreted by well-differenciated granulocytes and probably directly linked to membrane phospholipids.
Antibody Registry ID: AB_10129757 
Recommended Applications: FACS, Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ANTI-GRL(1) was deposited to the DSHB by Thomas, J.-L. (DSHB Hybridoma Product ANTI-GRL(1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • IP References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IP References
    FACS References
    Epitope Map References
    All References

    Identification in the chicken of GRL1 and GRL2: two granule proteins expressed on the surface of activated leukocytes.
    Le Douarin NM
    Experimental cell research 204.1 (1993 Jan): 156-66.

    Two proteins associated with secretory granule membranes identified in chicken regulated secretory cells.
    Gonatas N
    Journal of cell science 107 ( Pt 5). (1994 May): 1297-308.

    Novel monoclonal antibodies recognise guinea fowl thrombocytes.
    Oláh I
    Acta veterinaria Hungarica 57.2 (2009 Jun): 239-46.

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