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View product citations for antibody ANTI-GRL(1) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: leukocyte secretory granules, chicken
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Thomas, J.-L.
Isotype: MIgG3
Antigen Sequence:
Host Species: mouse
Depositors Institution: INSERM U495, Hopital de la Salpetriere
Positive Tested Species Reactivity: Chicken
Depositors Notes: Recognizes granulocytes, activated thrombocytes, acinar cells of the pancreas, and is a marker of secretory granules expressed on the plasma membrane of activated cells.
Antigen Molecular Weight: Apparent: 92 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: E15 bursa of Fabricius
Alternate Gene Names:
Alternate Antibody Name: I-A5
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: Cell surface epitope
Uniprot ID:  
Immunogen Sequence: Full length protein
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_10129757 
Additional Information: Stains intracytoplasmic granules of granulocytes and thrombocytes and are present on the surface of these cells as a result of exocytosis of the intracytoplasmic granules. GRL1 is a protein shed or dsecreted by well-differenciated granulocytes and probably directly linked to membrane phospholipids.
Recommended Applications: FACS, Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ANTI-GRL(1) was deposited to the DSHB by Thomas, J.-L. (DSHB Hybridoma Product ANTI-GRL(1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • IP References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IP References
    FACS References
    Epitope Map References
    All References

    Identification in the chicken of GRL1 and GRL2: two granule proteins expressed on the surface of activated leukocytes.
    Le Douarin NM
    Experimental cell research 204.1 (1993 Jan): 156-66.

    Two proteins associated with secretory granule membranes identified in chicken regulated secretory cells.
    Gonatas N
    Journal of cell science 107 ( Pt 5). (1994 May): 1297-308.

    Novel monoclonal antibodies recognise guinea fowl thrombocytes.
    Oláh I
    Acta veterinaria Hungarica 57.2 (2009 Jun): 239-46.

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