ASPL-TFE3 type 1

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SKU: ASPL-TFE3 type 1

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DSHB Data Sheet

Catalog Fields

Product Name/ID: ASPL-TFE3 type 1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2619584 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Synthetic peptide
Clone:
Immunogen Sequence: ASPL-TFE3 DPQQEQERERLPVSGNLLDVYSSQG KLH-conjugated
Myeloma Strain: NS1
Epitope Mapped: Yes
Antigen Name: Alveolar soft part sarcoma locus-transcription factor E3 fusion protein (ASPL-TFE3) type 1
Epitope Location or Sequence: aa 302-311 of ASPL & aa 296-310 of TFE3
Alternate Antigen Name:
Deposit Date: 1/31/2012
Antigen Molecular Weight:
Depositor: Vistica, D.T.
Antigen Sequence:
Depositor Institution: NCI
Antigen Species: Human
Depositor Notes: This antigen arises by chromosomal translocation to create fusion protein in a rare neoplasm found predominantly in children.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: To our knowledge, this antibody has not yet been used in a publication. Reference [PMID: 18176180] uses the same methods as those recommended by the depositor for this antibody. RRID:AB_2619584
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FFPE, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ASPL-TFE3 type 1 was deposited to the DSHB by Vistica, D.T. (DSHB Hybridoma Product ASPL-TFE3 type 1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication

    Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma.
    Shoemaker RH
    Journal of pediatric hematology/oncology 30.1 (2008 Jan): 46-52.

    WB References

    Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma.
    Shoemaker RH
    Journal of pediatric hematology/oncology 30.1 (2008 Jan): 46-52.

    IHC References

    Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma.
    Shoemaker RH
    Journal of pediatric hematology/oncology 30.1 (2008 Jan): 46-52.

    IP References

    Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma.
    Shoemaker RH
    Journal of pediatric hematology/oncology 30.1 (2008 Jan): 46-52.

    ELISA References

    Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma.
    Shoemaker RH
    Journal of pediatric hematology/oncology 30.1 (2008 Jan): 46-52.

    FFPE References

    Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma.
    Shoemaker RH
    Journal of pediatric hematology/oncology 30.1 (2008 Jan): 46-52.

    Epitope Map References

    Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma.
    Shoemaker RH
    Journal of pediatric hematology/oncology 30.1 (2008 Jan): 46-52.

    All References

    Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma.
    Shoemaker RH
    Journal of pediatric hematology/oncology 30.1 (2008 Jan): 46-52.

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