ASPL-TFE3 type 2

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$40.00
SKU: ASPL-TFE3 type 2
View product citations for antibody ASPL-TFE3 type 2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Alveolar soft part sarcoma locus-transcription factor E3 fusion protein (ASPL-TFE3) type 2
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Vistica, D.T.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: NCI
Positive Tested Species Reactivity: Human
Depositors Notes: This antigen arises by chromosomal translocation to create fusion protein in a rare neoplasm found predominantly in children.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Synthetic peptide
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: aa 302-311 of ASPL & aa 261-275 of TFE3
Uniprot ID:  
Immunogen Sequence: ASPL-TFE3 DPQQEQERERIDDVIDEIISLESSY KLH-conjugated
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2619585 
Additional Information: To our knowledge, this antibody has not yet been used in a publication. Reference [PMID: 18176180] uses the same methods as those recommended by the depositor for this antibody. RRID:AB_2619585
Recommended Applications: ELISA, FFPE, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ASPL-TFE3 type 2 was deposited to the DSHB by Vistica, D.T. (DSHB Hybridoma Product ASPL-TFE3 type 2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication

    Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma.
    Shoemaker RH
    Journal of pediatric hematology/oncology 30.1 (2008 Jan): 46-52.

    WB References

    Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma.
    Shoemaker RH
    Journal of pediatric hematology/oncology 30.1 (2008 Jan): 46-52.

    IHC References

    Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma.
    Shoemaker RH
    Journal of pediatric hematology/oncology 30.1 (2008 Jan): 46-52.

    IP References

    Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma.
    Shoemaker RH
    Journal of pediatric hematology/oncology 30.1 (2008 Jan): 46-52.

    ELISA References

    Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma.
    Shoemaker RH
    Journal of pediatric hematology/oncology 30.1 (2008 Jan): 46-52.

    FFPE References

    Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma.
    Shoemaker RH
    Journal of pediatric hematology/oncology 30.1 (2008 Jan): 46-52.

    Epitope Map References

    Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma.
    Shoemaker RH
    Journal of pediatric hematology/oncology 30.1 (2008 Jan): 46-52.

    All References

    Immunohistochemical discrimination between the ASPL-TFE3 fusion proteins of alveolar soft part sarcoma.
    Shoemaker RH
    Journal of pediatric hematology/oncology 30.1 (2008 Jan): 46-52.

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