Als9-2_9-A7
Catalog Fields
Clone ID/Product Name: Als9-2_9-A7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: ALS9-2
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2942064
Uniprot ID: A0A1D8PQ86
Entrez Gene ID: 3640281
Clonality: Monoclonal
Immunogen: Pichia pastoris GS115-expressed, His-tagged protein domains
Clone:
Immunogen Sequence: a.a. 18-328
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: Agglutinin-like sequence protein 9-2/ Als9-2
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/4/2022
Antigen Molecular Weight: 201.2 kDa
Depositor: Hoyer, L.L.
Antigen Sequence:
Depositor Institution: University of Illinois Urbana-Champaign
Antigen Species: C. albicans
Depositor Notes: C. albicans ALS9 has two common alleles (ALS9-1 and ALS9-2). The alleles encode proteins that are 84% identical in the N-terminal adhesive domain. Als9-1 is less involved in endothelial cell adhesion than the other isoform, Als9-2. Monoclonal antibodies deposited here differentiate between the Als9-1 and Als9-2 proteins.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: C. albicans
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Als9-2_9-A7 was deposited to the DSHB by Hoyer, L.L. (DSHB Hybridoma Product Als9-2_9-A7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
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