Anti-GFP [DSHB-12E6-Xi/Rabbit]
![Anti-GFP [DSHB-12E6-Xi/Rabbit]](https://dshb.biology.uiowa.edu/scs/img/2023_Logomark_Circle-Histo.png?resizeid=3&resizeh=600&resizew=600)
Catalog Fields
Clone ID/Product Name: Anti-GFP [DSHB-12E6-Xi/Rabbit]
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: GFP
Ab Isotype: Rabbit IgG
Gene Name:
Antibody Registry ID: AB_3696611
Uniprot ID: P42212
Clonality: Monoclonal
Immunogen: Recombinant GFP expressed in E. coli
Clone:
Immunogen Sequence: Full recombinant protein
Myeloma Strain:
Epitope Mapped: No
Antigen Name: green fluorescent protein (GFP)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/15/2025
Antigen Molecular Weight: 26.8 kDa
Depositor: DSHB
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: Aequorea victoria
Depositor Notes: This is a chimeric recombinant rabbit IgG antibody; anti-GFP [DSHB-12E6] does not work for western blot.
Host Species: rabbit
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity:
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, ELISA, FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Anti-GFP [DSHB-12E6-Xi/Rabbit] was deposited to the DSHB by DSHB (DSHB Hybridoma Product Anti-GFP [DSHB-12E6-Xi/Rabbit])
Storage and Handling Recommendations
Although many cell products are maintained at 4?C for years without loss of activity, shelf-life at 4?C is highly variable. For immediate use, short term storage at 4?C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 µl for freezing at -20?C or -80?C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 µg/ml. For western blots, the recommended concentration range is 0.2-0.5 µg/ml.
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