B30

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SKU: B30-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: B30
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgM
Gene Symbol:
Antibody Registry ID: AB_531879 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: rat mid- and hindbrain (whole cells)
Clone:
Immunogen Sequence:
Myeloma Strain: FOX-NY
Epitope Mapped: No
Antigen Name: neuronal, mesencephalic trigeminal cell surface marker
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 10/24/1990
Antigen Molecular Weight:
Depositor: Gilbert, W. / Stainier, D.
Antigen Sequence:
Depositor Institution: Max Planck Institute for Heart & Lung Research
Antigen Species: rat
Depositor Notes: gangliosides. Immunoblotting note: 2 bands on TLC. IHC note: yes, on fresh tissue.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Mouse, Rat
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
B30 was deposited to the DSHB by Gilbert, W. / Stainier, D. (DSHB Hybridoma Product B30)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    The monoclonal antibody B30 recognizes a specific neuronal cell surface antigen in the developing mesencephalic trigeminal nucleus of the mouse.
    Gilbert W
    The Journal of neuroscience : the official journal of the Society for Neuroscience 9.7 (1989 Jul): 2468-85.

    All References

    The monoclonal antibody B30 recognizes a specific neuronal cell surface antigen in the developing mesencephalic trigeminal nucleus of the mouse.
    Gilbert W
    The Journal of neuroscience : the official journal of the Society for Neuroscience 9.7 (1989 Jul): 2468-85.

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