BB8/384.1
Catalog Fields
Clone ID/Product Name: BB8/384.1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Actn
Ab Isotype: RIgG2a
Gene Name:
Antibody Registry ID: AB_2889802
Uniprot ID: P18091
Entrez Gene ID: 31166
Clonality: Monoclonal
Immunogen: Myofibril preparation
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: Y3Agl.2.3.
Epitope Mapped: No
Antigen Name: Alpha-actinin
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 12/10/2019
Antigen Molecular Weight: 107 kDa
Depositor: Bullard, Belinda
Antigen Sequence:
Depositor Institution: University of York
Antigen Species: Lethocerus indicus & Drosophila Melanogaster
Depositor Notes: This antibody is also known as MAC 276
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila, Lethocerus indicus
Additional Information: RRID: AB_2889802, FBgn0000667.
Recommended Applications:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BB8/384.1 was deposited to the DSHB by Bullard, Belinda (DSHB Hybridoma Product BB8/384.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
1 Reference
Initial Publication
Identification and localization of high molecular weight proteins in insect flight and leg muscle.
Bullard B
The EMBO journal 9.11 (1990 Nov): 3459-67.