Antigen: Botrytis cinerea
Hybridoma Cells Available: No
Antigen Species: Botrytis cinerea
Depositor: Dewey, F.M.
Host Species: mouse
Depositors Institution: University of Oxford
Positive Tested Species Reactivity: Botrytis cinerea
Depositors Notes: Water soluble, thermostable glycoprotein that is not metabolized during fermentation of infected grape juice. Near-genus specific, recognizes all species of Botrytis and species of related genus Sclerotinia(fungi) plus Magnapothe grisea, but does not recognize other fungi including yeasts or extracts from uninfected plants. Cell binding: binds to extracellular matrix of hyphal walls species of Botrytis and Sclerotinia (fungi), does not bind to asexual spores (conidia) or sexual spores (ascospores). Immunohistology: has been used in immunofluorescence and TEM studies to visualize the fungal hyphae in vitro and in vivo. IHC note: binding site is carbohydrate containing rhamnose
Predicted Species Reactivity:
Immunogen: PBS cell surface washings of plate culture of Botrytis cinerea + hybridoma supernatant from nonspecific cell line BC-EC7 in 1:1 ratio (Meyer & Dewey, 2000)
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence:
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BC-12.CA4 was deposited to the DSHB by Dewey, F.M. (DSHB Hybridoma Product BC-12.CA4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.