Product Name/ID: C17
Available to For-Profits: Yes
Alternate Antibody Name:
Ab Isotype: MIgG1
Antibody Registry ID: AB_2617206
Immunogen: rat Reichert’s membrane
Myeloma Strain: NS1
Epitope Mapped: No
Antigen Name: heparan sulfate proteoglycan (basement membrane)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 7/22/1992
Antigen Molecular Weight: >400 kDa
Depositor: Sanes, J.R.
Depositor Institution: Harvard University
Antigen Species: rat
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Rat
Recommended Applications: Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C17 was deposited to the DSHB by Sanes, J.R. (DSHB Hybridoma Product C17)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).