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SKU: C17
View product citations for antibody C17 on CiteAb

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Available: 25

DSHB Data Sheet

Catalog Fields

Antigen: heparan sulfate proteoglycan (basement membrane)
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Sanes, J.R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Harvard University
Positive Tested Species Reactivity: Rat
Depositors Notes:
Antigen Molecular Weight: >400 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: rat Reichert’s membrane
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2617206 
Additional Information:
Recommended Applications: Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C17 was deposited to the DSHB by Sanes, J.R. (DSHB Hybridoma Product C17)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    The basal lamina of the neuromuscular junction.
    Chiu AY
    Cold Spring Harbor symposia on quantitative biology 48 Pt 2. (1983): 667-78.

    All References

    The basal lamina of the neuromuscular junction.
    Chiu AY
    Cold Spring Harbor symposia on quantitative biology 48 Pt 2. (1983): 667-78.

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