C3/1

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$40.00
SKU: C3/1
View product citations for antibody C3/1 on CiteAb

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Available: 34

DSHB Data Sheet

Catalog Fields

Antigen: Myoblast and muscle satellite cell marker
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fambrough, D.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins University, Baltimore
Positive Tested Species Reactivity: Chick
Depositors Notes: This antibody also binds to peripheral Schwann cell, glia and non-neuronal cells derived from embryonic spinal cord.
Antigen Molecular Weight: 38 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen:
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0 Ag-14
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_531889 
Additional Information: RRID:AB_531889
Recommended Applications: Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C3/1 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product C3/1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References
    IHC References

    Insulin-like growth factor I stimulates myoblast expansion and myofiber development in the limb.
    Hannon K
    Developmental dynamics : an official publication of the American Association of Anatomists 223.1 (2002 Jan): 12-23.

    IP References
    All References

    Insulin-like growth factor I stimulates myoblast expansion and myofiber development in the limb.
    Hannon K
    Developmental dynamics : an official publication of the American Association of Anatomists 223.1 (2002 Jan): 12-23.

    Characterization of a plasma membrane glycoprotein common to myoblasts, skeletal muscle satellite cells, and glia.
    Fambrough DM
    Developmental biology 100.2 (1983 Dec): 464-77.

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