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SKU: CBR LFA-1/2-s

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Available: 11

DSHB Data Sheet

Catalog Fields

Product Name/ID: CBR LFA-1/2
Available to For-Profits: No
Alternate Antibody Name:
Gene Name: ITGB2
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_3094516 
Uniprot ID: P05107 
Entrez Gene ID: 3689 
Clonality: Monoclonal
Immunogen: LFA-1 purified by TS2/4 affinity chromatrography.
Immunogen Sequence: Full length sequence
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: Yes
Antigen Name: Integrin beta-2 (CD18)
Epitope Location or Sequence: Cytoplasmic domains involving residues 534, 536, 541, 543 and 546 in cysteine-rich repeat 3.
Alternate Antigen Name:
Deposit Date: 2/21/2024
Antigen Molecular Weight: 84.7 kDa (core protein) 90-95 kDa (glycoprotein)
Depositor: Springer, T.A.
Antigen Sequence:
Depositor Institution: Boston Children’s Hospital, Harvard University
Antigen Species: Human
Depositor Notes: CD18 is associated with CD11a to form LFA-1 which binds to CD54, CD102 and CD50.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: mAb CBR LFA-1/2 bindsing activates LFA-1 signaling.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Chromatin Immunoprecipitation, ELISA, FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CBR LFA-1/2 was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product CBR LFA-1/2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

2 References

  • Initial Publication
  • Epitope Map References
  • All References
  • Initial Publication
    Epitope Map References
    All References

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