CG1

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$40.00
SKU: CG1
View product citations for antibody CG1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: tropomyosin, TPM2
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Lin, J.J.-C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Chicken, Ferret, Human, Rodent
Depositors Notes:
Antigen Molecular Weight: Apparent: 36 kDa (Tm1), 30 kDa (Tm4)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: TPM2
Immunogen: purified chicken gizzard tropomyosin
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: P19352 
Immunogen Sequence: Full length protein
Entrez Gene ID: 396430 
Additional Characterization:  
Antibody Registry ID: AB_10573117 
Additional Information: CG1 recognizes the fast-migrating component of gizzard tropomyosin, skeletal beta-tropomyosin and alpha-tropomyosin, and chicken embryo fibroblasts tropomyosin. Recognizes Human and rat Tm1 (encoded by TPM2) and cross-reacts with Tm4 (TPM4) on western blots [PMID: 22069507], and has been reported to be specific to Tpm2.1 [PMID: 27112294; 26147585].
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CG1 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product CG1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • FACS References
  • All References
  • Initial Publication
    IF References
    WB References

    Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application.
    Lin JL
    Hybridoma 4.3 (1985 Fall): 223-42.

    Probing the role of nonmuscle tropomyosin isoforms in intracellular granule movement by microinjection of monoclonal antibodies.
    Lin JJ
    The Journal of cell biology 109.3 (1989 Sep): 1141-52.

    Motility-dependence of the heterogenous staining of culture cells by a monoclonal anti-tropomyosin antibody.
    Lin JJ
    The Journal of cell biology 106.2 (1988 Feb): 385-93.

    Expression of smooth muscle and nonmuscle tropomyosins in Escherichia coli and characterization of bacterially produced tropomyosins.
    Lin JJ
    Biochimica et biophysica acta 1162.3 (1993 Mar 26): 255-65.

    Tropomyosin isoforms in intestinal mucosa: production of autoantibodies to tropomyosin isoforms in ulcerative colitis.
    Das KM
    Gastroenterology 114.5 (1998 May): 912-22.

    Stable incorporation of α-smooth muscle actin into stress fibers is dependent on specific tropomyosin isoforms.
    Chaponnier C
    Cytoskeleton (Hoboken, N.J.) 72.6 (2015 Jun): 257-67.

    Physiological contractility of cardiomyocytes in the wall of mouse and rat azygos vein.
    Jin JP
    American journal of physiology. Cell physiology 306.7 (2014 Apr 1): C697-704.

    Autoimmunity against human tropomyosin isoforms in ulcerative colitis: localization of specific human tropomyosin isoforms in the intestine and extraintestinal organs.
    Das KM
    Inflammatory bowel diseases 12.11 (2006 Nov): 1036-43.

    Accumulation of tropomyosin isoform 5 at the infection sites of host cells during Cryptosporidium invasion.
    Lin JJ
    Parasitology research 99.1 (2006 Jun): 45-54.

    Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells.
    Morgan KG
    American journal of physiology. Cell physiology 300.6 (2011 Jun): C1356-65.

    IHC References
    IP References
    FB References
    FACS References

    Externalization of tropomyosin isoform 5 in colon epithelial cells.
    Das KM
    Clinical and experimental immunology 118.2 (1999 Nov): 219-27.

    All References

    Autoimmunity against human tropomyosin isoforms in ulcerative colitis: localization of specific human tropomyosin isoforms in the intestine and extraintestinal organs.
    Das KM
    Inflammatory bowel diseases 12.11 (2006 Nov): 1036-43.

    Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application.
    Lin JL
    Hybridoma 4.3 (1985 Fall): 223-42.

    Motility-dependence of the heterogenous staining of culture cells by a monoclonal anti-tropomyosin antibody.
    Lin JJ
    The Journal of cell biology 106.2 (1988 Feb): 385-93.

    Tropomyosin isoforms and reagents.
    Gunning PW
    Bioarchitecture 1.4 (2011 Jul): 135-164.

    Tropomyosin Promotes Lamellipodial Persistence by Collaborating with Arp2/3 at the Leading Edge.
    Gunning PW
    Current biology : CB 26.10 (2016 May 23): 1312-8.

    Stable incorporation of α-smooth muscle actin into stress fibers is dependent on specific tropomyosin isoforms.
    Chaponnier C
    Cytoskeleton (Hoboken, N.J.) 72.6 (2015 Jun): 257-67.

    Probing the role of nonmuscle tropomyosin isoforms in intracellular granule movement by microinjection of monoclonal antibodies.
    Lin JJ
    The Journal of cell biology 109.3 (1989 Sep): 1141-52.

    Expression of smooth muscle and nonmuscle tropomyosins in Escherichia coli and characterization of bacterially produced tropomyosins.
    Lin JJ
    Biochimica et biophysica acta 1162.3 (1993 Mar 26): 255-65.

    Tropomyosin isoforms in intestinal mucosa: production of autoantibodies to tropomyosin isoforms in ulcerative colitis.
    Das KM
    Gastroenterology 114.5 (1998 May): 912-22.

    Physiological contractility of cardiomyocytes in the wall of mouse and rat azygos vein.
    Jin JP
    American journal of physiology. Cell physiology 306.7 (2014 Apr 1): C697-704.

    Accumulation of tropomyosin isoform 5 at the infection sites of host cells during Cryptosporidium invasion.
    Lin JJ
    Parasitology research 99.1 (2006 Jun): 45-54.

    Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells.
    Morgan KG
    American journal of physiology. Cell physiology 300.6 (2011 Jun): C1356-65.

    Externalization of tropomyosin isoform 5 in colon epithelial cells.
    Das KM
    Clinical and experimental immunology 118.2 (1999 Nov): 219-27.

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