CG3
Catalog Fields
Clone ID/Product Name: CG3
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: TPM3
Ab Isotype: MIgM
Gene Name:
Antibody Registry ID: AB_10572305
Entrez Gene ID: 770103
Clonality: Monoclonal
Immunogen: purified chicken gizzard tropomyosin
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS1
Epitope Mapped: Yes
Antigen Name: Tropomyosin, alpha-1 chain
Epitope Location or Sequence: In Human, aa 29-44 encoded by exon 1b of TPM3 (AERLQREVEGERRAREQ)
Alternate Antigen Name:
Deposit Date: 7/17/2009
Antigen Molecular Weight: Apparent: 32 kDa
Depositor: Lin, J.J.-C.
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: Chicken
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Human, Rodent
Additional Information: CG3 recognizes all chicken tropomyosin isoforms [PMID: 3899907]. CG3 is specific to all cytoskeletal products from the TPM3 gene [PMID: 22069507], both human and mouse. Can also detect the striated muscle isoform encoded by this gene by cross reacting with theTPM3 exons 1a and 2b products.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CG3 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product CG3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
26 References
Initial Publication
Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application.
Lin JL
Hybridoma 4.3 (1985 Fall): 223-42.