CG3

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$40.00
SKU: CG3
View product citations for antibody CG3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: tropomyosin, TPM3
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Lin, J.J.-C.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Chicken, Human, Rodent
Depositors Notes:
Antigen Molecular Weight: Apparent: 32 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: TPM3
Immunogen: purified chicken gizzard tropomyosin
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: In Human, aa 29-44 encoded by exon 1b of TPM3 (AERLQREVEGERRAREQ)
Uniprot ID:  
Immunogen Sequence: Full length protein
Entrez Gene ID: 770103 
Additional Characterization:  
Antibody Registry ID: AB_10572305 
Additional Information: CG3 recognizes all chicken tropomyosin isoforms [PMID: 3899907]. CG3 is specific to all cytoskeletal products from the TPM3 gene [PMID: 22069507], both human and mouse. Can also detect the striated muscle isoform encoded by this gene by cross reacting with theTPM3 exons 1a and 2b products.
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CG3 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product CG3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

26 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application.
    Lin JL
    Hybridoma 4.3 (1985 Fall): 223-42.

    Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis.
    Lin JJ
    The Journal of cell biology 129.3 (1995 May): 697-708.

    Tropomyosin isoforms and reagents.
    Gunning PW
    Bioarchitecture 1.4 (2011 Jul): 135-164.

    Hypoxia alters the recruitment of tropomyosins into the actin stress fibres of neuroblastoma cells.
    Stehn JR
    BMC cancer 15. (2015 Oct 16): 712.

    Deletion of calponin 2 in macrophages alters cytoskeleton-based functions and attenuates the development of atherosclerosis.
    Jin JP
    Journal of molecular and cellular cardiology 99. (2016 Oct): 87-99.

    Identification of a human mitochondrial RNA that promotes tropomyosin synthesis and myocardial differentiation.
    Lemanski LF
    In vitro cellular & developmental biology. Animal 51.3 (2015 Mar): 273-80.

    Differential localization of tropomyosin isoforms in cultured nonmuscle cells.
    Lin JL
    The Journal of cell biology 107.2 (1988 Aug): 563-72.

    Overexpression of human fibroblast caldesmon fragment containing actin-, Ca++/calmodulin-, and tropomyosin-binding domains stabilizes endogenous tropomyosin and microfilaments.
    Lin JJ
    The Journal of cell biology 125.2 (1994 Apr): 359-68.

    Sorting of tropomyosin isoforms in synchronised NIH 3T3 fibroblasts: evidence for distinct microfilament populations.
    Gunning P
    Cell motility and the cytoskeleton 47.3 (2000 Nov): 189-208.

    Sorting of a nonmuscle tropomyosin to a novel cytoskeletal compartment in skeletal muscle results in muscular dystrophy.
    Hardeman EC
    The Journal of cell biology 166.5 (2004 Aug 30): 685-96.

    Accumulation of tropomyosin isoform 5 at the infection sites of host cells during Cryptosporidium invasion.
    Lin JJ
    Parasitology research 99.1 (2006 Jun): 45-54.

    Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edge.
    Hitchcock-DeGregori SE
    Journal of cell science 115.Pt 23 (2002 Dec 1): 4649-60.

    WB References

    Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application.
    Lin JL
    Hybridoma 4.3 (1985 Fall): 223-42.

    Probing the role of nonmuscle tropomyosin isoforms in intracellular granule movement by microinjection of monoclonal antibodies.
    Lin JJ
    The Journal of cell biology 109.3 (1989 Sep): 1141-52.

    Tropomyosin isoform expression and phosphorylation in the human heart in health and disease.
    Ward DG
    Journal of muscle research and cell motility 34.3-4 (2013 Aug): 189-97.

    Motility-dependence of the heterogenous staining of culture cells by a monoclonal anti-tropomyosin antibody.
    Lin JJ
    The Journal of cell biology 106.2 (1988 Feb): 385-93.

    Tropomyosin isoforms and reagents.
    Gunning PW
    Bioarchitecture 1.4 (2011 Jul): 135-164.

    Tropomyosin isoforms in intestinal mucosa: production of autoantibodies to tropomyosin isoforms in ulcerative colitis.
    Das KM
    Gastroenterology 114.5 (1998 May): 912-22.

    Autoimmunity against human tropomyosin isoforms in ulcerative colitis: localization of specific human tropomyosin isoforms in the intestine and extraintestinal organs.
    Das KM
    Inflammatory bowel diseases 12.11 (2006 Nov): 1036-43.

    Differential localization of tropomyosin isoforms in cultured nonmuscle cells.
    Lin JL
    The Journal of cell biology 107.2 (1988 Aug): 563-72.

    Overexpression of human fibroblast caldesmon fragment containing actin-, Ca++/calmodulin-, and tropomyosin-binding domains stabilizes endogenous tropomyosin and microfilaments.
    Lin JJ
    The Journal of cell biology 125.2 (1994 Apr): 359-68.

    Externalization of tropomyosin isoform 5 in colon epithelial cells.
    Das KM
    Clinical and experimental immunology 118.2 (1999 Nov): 219-27.

    Sorting of tropomyosin isoforms in synchronised NIH 3T3 fibroblasts: evidence for distinct microfilament populations.
    Gunning P
    Cell motility and the cytoskeleton 47.3 (2000 Nov): 189-208.

    Tropomyosin isoform 5b is expressed in human erythrocytes: implications of tropomodulin-TM5 or tropomodulin-TM5b complexes in the protofilament and hexagonal organization of membrane skeletons.
    Mehrpouryan M
    Blood 95.4 (2000 Feb 15): 1473-80.

    Tropomodulin-binding site mapped to residues 7-14 at the N-terminal heptad repeats of tropomyosin isoform 5.
    Sung LA
    Archives of biochemistry and biophysics 378.1 (2000 Jun 1): 16-24.

    Sorting of a nonmuscle tropomyosin to a novel cytoskeletal compartment in skeletal muscle results in muscular dystrophy.
    Hardeman EC
    The Journal of cell biology 166.5 (2004 Aug 30): 685-96.

    Tissue-specific tropomyosin isoform composition.
    Gunning PW
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 53.5 (2005 May): 557-70.

    Accumulation of tropomyosin isoform 5 at the infection sites of host cells during Cryptosporidium invasion.
    Lin JJ
    Parasitology research 99.1 (2006 Jun): 45-54.

    Changes in protein profiles during course of experimental glomerulonephritis.
    Budisavljevic MN
    American journal of physiology. Renal physiology 296.1 (2009 Jan): F186-93.

    Tropomodulin-binding site mapped to residues 7-14 at the N-terminal heptad repeats of tropomyosin isoform 5.
    Sung LA
    Archives of biochemistry and biophysics 378.1 (2000 Jun 1): 16-24.

    Tropomyosin isoform 5b is expressed in human erythrocytes: implications of tropomodulin-TM5 or tropomodulin-TM5b complexes in the protofilament and hexagonal organization of membrane skeletons.
    Mehrpouryan M
    Blood 95.4 (2000 Feb 15): 1473-80.

    Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edge.
    Hitchcock-DeGregori SE
    Journal of cell science 115.Pt 23 (2002 Dec 1): 4649-60.

    Alterations in tropomyosin isoform expression in human transitional cell carcinoma of the urinary bladder.
    Helfman DM
    International journal of cancer 110.3 (2004 Jun 20): 368-73.

    Renal ischemia induces tropomyosin dissociation-destabilizing microvilli microfilaments.
    Molitoris BA
    American journal of physiology. Renal physiology 286.5 (2004 May): F988-96.

    IHC References
    IP References
    FACS References

    Externalization of tropomyosin isoform 5 in colon epithelial cells.
    Das KM
    Clinical and experimental immunology 118.2 (1999 Nov): 219-27.

    Epitope Map References

    Tropomyosin isoforms and reagents.
    Gunning PW
    Bioarchitecture 1.4 (2011 Jul): 135-164.

    All References

    Autoimmunity against human tropomyosin isoforms in ulcerative colitis: localization of specific human tropomyosin isoforms in the intestine and extraintestinal organs.
    Das KM
    Inflammatory bowel diseases 12.11 (2006 Nov): 1036-43.

    Sorting of a nonmuscle tropomyosin to a novel cytoskeletal compartment in skeletal muscle results in muscular dystrophy.
    Hardeman EC
    The Journal of cell biology 166.5 (2004 Aug 30): 685-96.

    Accumulation of tropomyosin isoform 5 at the infection sites of host cells during Cryptosporidium invasion.
    Lin JJ
    Parasitology research 99.1 (2006 Jun): 45-54.

    Tropomyosins in human diseases: ulcerative colitis.
    Bajpai M
    Advances in experimental medicine and biology 644. (2008): 158-67.

    Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application.
    Lin JL
    Hybridoma 4.3 (1985 Fall): 223-42.

    Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis.
    Lin JJ
    The Journal of cell biology 129.3 (1995 May): 697-708.

    Tropomyosin isoforms and reagents.
    Gunning PW
    Bioarchitecture 1.4 (2011 Jul): 135-164.

    Hypoxia alters the recruitment of tropomyosins into the actin stress fibres of neuroblastoma cells.
    Stehn JR
    BMC cancer 15. (2015 Oct 16): 712.

    Deletion of calponin 2 in macrophages alters cytoskeleton-based functions and attenuates the development of atherosclerosis.
    Jin JP
    Journal of molecular and cellular cardiology 99. (2016 Oct): 87-99.

    Identification of a human mitochondrial RNA that promotes tropomyosin synthesis and myocardial differentiation.
    Lemanski LF
    In vitro cellular & developmental biology. Animal 51.3 (2015 Mar): 273-80.

    Differential localization of tropomyosin isoforms in cultured nonmuscle cells.
    Lin JL
    The Journal of cell biology 107.2 (1988 Aug): 563-72.

    Overexpression of human fibroblast caldesmon fragment containing actin-, Ca++/calmodulin-, and tropomyosin-binding domains stabilizes endogenous tropomyosin and microfilaments.
    Lin JJ
    The Journal of cell biology 125.2 (1994 Apr): 359-68.

    Sorting of tropomyosin isoforms in synchronised NIH 3T3 fibroblasts: evidence for distinct microfilament populations.
    Gunning P
    Cell motility and the cytoskeleton 47.3 (2000 Nov): 189-208.

    Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edge.
    Hitchcock-DeGregori SE
    Journal of cell science 115.Pt 23 (2002 Dec 1): 4649-60.

    Probing the role of nonmuscle tropomyosin isoforms in intracellular granule movement by microinjection of monoclonal antibodies.
    Lin JJ
    The Journal of cell biology 109.3 (1989 Sep): 1141-52.

    Tropomyosin isoform expression and phosphorylation in the human heart in health and disease.
    Ward DG
    Journal of muscle research and cell motility 34.3-4 (2013 Aug): 189-97.

    Motility-dependence of the heterogenous staining of culture cells by a monoclonal anti-tropomyosin antibody.
    Lin JJ
    The Journal of cell biology 106.2 (1988 Feb): 385-93.

    Tropomyosin isoforms in intestinal mucosa: production of autoantibodies to tropomyosin isoforms in ulcerative colitis.
    Das KM
    Gastroenterology 114.5 (1998 May): 912-22.

    Externalization of tropomyosin isoform 5 in colon epithelial cells.
    Das KM
    Clinical and experimental immunology 118.2 (1999 Nov): 219-27.

    Tropomyosin isoform 5b is expressed in human erythrocytes: implications of tropomodulin-TM5 or tropomodulin-TM5b complexes in the protofilament and hexagonal organization of membrane skeletons.
    Mehrpouryan M
    Blood 95.4 (2000 Feb 15): 1473-80.

    Tropomodulin-binding site mapped to residues 7-14 at the N-terminal heptad repeats of tropomyosin isoform 5.
    Sung LA
    Archives of biochemistry and biophysics 378.1 (2000 Jun 1): 16-24.

    Tissue-specific tropomyosin isoform composition.
    Gunning PW
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 53.5 (2005 May): 557-70.

    Changes in protein profiles during course of experimental glomerulonephritis.
    Budisavljevic MN
    American journal of physiology. Renal physiology 296.1 (2009 Jan): F186-93.

    Alterations in tropomyosin isoform expression in human transitional cell carcinoma of the urinary bladder.
    Helfman DM
    International journal of cancer 110.3 (2004 Jun 20): 368-73.

    Renal ischemia induces tropomyosin dissociation-destabilizing microvilli microfilaments.
    Molitoris BA
    American journal of physiology. Renal physiology 286.5 (2004 May): F988-96.

    In vitro functional characterization of bacterially expressed human fibroblast tropomyosin isoforms and their chimeric mutants.
    Lin JJ
    Cell motility and the cytoskeleton 26.3 (1993): 248-61.

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