CGbeta6
Catalog Fields
Antigen: Tropomyosin, alpha-1 chain
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Lin, J.J.-C.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Chicken, Human, Rodent
Depositors Notes:
Predicted Species Reactivity:
Gene: TPM1
Immunogen: purified chicken gizzard tropomyosin
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: C-terminal end of Human Tm2 and Tm3 (TPM1)
Uniprot ID: P04268
Immunogen Sequence: Full length protein
Entrez Gene ID: 396366
Antibody Registry ID: AB_10573118
Additional Information: CGbeta6 recognizes the slow-migrating component of gizzard tropomyosin and cross-reacts with chicken embryo fibroblasts tropomyosin, particularly isoforms a, b, 1, and 2, but does not recognize tropomyosin isoforms from striated muscle. Recognizes Human and rat Tm2 and Tm3 (encoded by TPM1) and rat Tm5a (TPM1) [PMID: 22069507].
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CGbeta6 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product CGbeta6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
19 References
Initial Publication
Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application.
Lin JL
Hybridoma 4.3 (1985 Fall): 223-42.