CGbeta6

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$40.00
SKU: CGbeta6
View product citations for antibody CGbeta6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: tropomyosin, TPM1
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Lin, J.J.-C.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Chicken, Human, Rodent
Depositors Notes:
Antigen Molecular Weight: Apparent: 34kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: TPM1
Immunogen: purified chicken gizzard tropomyosin
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: C-terminal end of Human Tm2 and Tm3 (TPM1)
Uniprot ID: P04268 
Immunogen Sequence: Full length protein
Entrez Gene ID: 396366 
Additional Characterization:  
Antibody Registry ID: AB_10573118 
Additional Information: CGbeta6 recognizes the slow-migrating component of gizzard tropomyosin and cross-reacts with chicken embryo fibroblasts tropomyosin, particularly isoforms a, b, 1, and 2, but does not recognize tropomyosin isoforms from striated muscle. Recognizes Human and rat Tm2 and Tm3 (encoded by TPM1) and rat Tm5a (TPM1) [PMID: 22069507].
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CGbeta6 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product CGbeta6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

19 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis.
    Lin JJ
    The Journal of cell biology 129.3 (1995 May): 697-708.

    Tropomyosin isoforms and reagents.
    Gunning PW
    Bioarchitecture 1.4 (2011 Jul): 135-164.

    Stable incorporation of α-smooth muscle actin into stress fibers is dependent on specific tropomyosin isoforms.
    Chaponnier C
    Cytoskeleton (Hoboken, N.J.) 72.6 (2015 Jun): 257-67.

    Hypoxia alters the recruitment of tropomyosins into the actin stress fibres of neuroblastoma cells.
    Stehn JR
    BMC cancer 15. (2015 Oct 16): 712.

    Differential localization of tropomyosin isoforms in cultured nonmuscle cells.
    Lin JL
    The Journal of cell biology 107.2 (1988 Aug): 563-72.

    Human tropomyosin isoforms in the regulation of cytoskeleton functions.
    McCrae KR
    Advances in experimental medicine and biology 644. (2008): 201-22.

    Tropomyosin and caldesmon regulate cytokinesis speed and membrane stability during cell division.
    Lin JJ
    Archives of biochemistry and biophysics 456.2 (2006 Dec 15): 161-74.

    A high molecular mass non-muscle tropomyosin isoform stimulates retrograde organelle transport.
    Wang YL
    Journal of cell science 109 ( Pt 5). (1996 May): 981-9.

    WB References

    Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application.
    Lin JL
    Hybridoma 4.3 (1985 Fall): 223-42.

    Probing the role of nonmuscle tropomyosin isoforms in intracellular granule movement by microinjection of monoclonal antibodies.
    Lin JJ
    The Journal of cell biology 109.3 (1989 Sep): 1141-52.

    Motility-dependence of the heterogenous staining of culture cells by a monoclonal anti-tropomyosin antibody.
    Lin JJ
    The Journal of cell biology 106.2 (1988 Feb): 385-93.

    Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis.
    Lin JJ
    The Journal of cell biology 129.3 (1995 May): 697-708.

    Expression of smooth muscle and nonmuscle tropomyosins in Escherichia coli and characterization of bacterially produced tropomyosins.
    Lin JJ
    Biochimica et biophysica acta 1162.3 (1993 Mar 26): 255-65.

    Tropomyosin isoforms in intestinal mucosa: production of autoantibodies to tropomyosin isoforms in ulcerative colitis.
    Das KM
    Gastroenterology 114.5 (1998 May): 912-22.

    Stable incorporation of α-smooth muscle actin into stress fibers is dependent on specific tropomyosin isoforms.
    Chaponnier C
    Cytoskeleton (Hoboken, N.J.) 72.6 (2015 Jun): 257-67.

    Hypoxia alters the recruitment of tropomyosins into the actin stress fibres of neuroblastoma cells.
    Stehn JR
    BMC cancer 15. (2015 Oct 16): 712.

    Physiological contractility of cardiomyocytes in the wall of mouse and rat azygos vein.
    Jin JP
    American journal of physiology. Cell physiology 306.7 (2014 Apr 1): C697-704.

    Autoimmunity against human tropomyosin isoforms in ulcerative colitis: localization of specific human tropomyosin isoforms in the intestine and extraintestinal organs.
    Das KM
    Inflammatory bowel diseases 12.11 (2006 Nov): 1036-43.

    Differential localization of tropomyosin isoforms in cultured nonmuscle cells.
    Lin JL
    The Journal of cell biology 107.2 (1988 Aug): 563-72.

    Accumulation of tropomyosin isoform 5 at the infection sites of host cells during Cryptosporidium invasion.
    Lin JJ
    Parasitology research 99.1 (2006 Jun): 45-54.

    Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edge.
    Hitchcock-DeGregori SE
    Journal of cell science 115.Pt 23 (2002 Dec 1): 4649-60.

    Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells.
    Morgan KG
    American journal of physiology. Cell physiology 300.6 (2011 Jun): C1356-65.

    IHC References
    IP References
    Epitope Map References

    Tropomyosin isoforms and reagents.
    Gunning PW
    Bioarchitecture 1.4 (2011 Jul): 135-164.

    All References

    Autoimmunity against human tropomyosin isoforms in ulcerative colitis: localization of specific human tropomyosin isoforms in the intestine and extraintestinal organs.
    Das KM
    Inflammatory bowel diseases 12.11 (2006 Nov): 1036-43.

    Tropomyosins in human diseases: ulcerative colitis.
    Bajpai M
    Advances in experimental medicine and biology 644. (2008): 158-67.

    Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis.
    Lin JJ
    The Journal of cell biology 129.3 (1995 May): 697-708.

    Tropomyosin isoforms and reagents.
    Gunning PW
    Bioarchitecture 1.4 (2011 Jul): 135-164.

    Stable incorporation of α-smooth muscle actin into stress fibers is dependent on specific tropomyosin isoforms.
    Chaponnier C
    Cytoskeleton (Hoboken, N.J.) 72.6 (2015 Jun): 257-67.

    Hypoxia alters the recruitment of tropomyosins into the actin stress fibres of neuroblastoma cells.
    Stehn JR
    BMC cancer 15. (2015 Oct 16): 712.

    Differential localization of tropomyosin isoforms in cultured nonmuscle cells.
    Lin JL
    The Journal of cell biology 107.2 (1988 Aug): 563-72.

    Human tropomyosin isoforms in the regulation of cytoskeleton functions.
    McCrae KR
    Advances in experimental medicine and biology 644. (2008): 201-22.

    Tropomyosin and caldesmon regulate cytokinesis speed and membrane stability during cell division.
    Lin JJ
    Archives of biochemistry and biophysics 456.2 (2006 Dec 15): 161-74.

    A high molecular mass non-muscle tropomyosin isoform stimulates retrograde organelle transport.
    Wang YL
    Journal of cell science 109 ( Pt 5). (1996 May): 981-9.

    Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application.
    Lin JL
    Hybridoma 4.3 (1985 Fall): 223-42.

    Probing the role of nonmuscle tropomyosin isoforms in intracellular granule movement by microinjection of monoclonal antibodies.
    Lin JJ
    The Journal of cell biology 109.3 (1989 Sep): 1141-52.

    Motility-dependence of the heterogenous staining of culture cells by a monoclonal anti-tropomyosin antibody.
    Lin JJ
    The Journal of cell biology 106.2 (1988 Feb): 385-93.

    Expression of smooth muscle and nonmuscle tropomyosins in Escherichia coli and characterization of bacterially produced tropomyosins.
    Lin JJ
    Biochimica et biophysica acta 1162.3 (1993 Mar 26): 255-65.

    Tropomyosin isoforms in intestinal mucosa: production of autoantibodies to tropomyosin isoforms in ulcerative colitis.
    Das KM
    Gastroenterology 114.5 (1998 May): 912-22.

    Physiological contractility of cardiomyocytes in the wall of mouse and rat azygos vein.
    Jin JP
    American journal of physiology. Cell physiology 306.7 (2014 Apr 1): C697-704.

    Accumulation of tropomyosin isoform 5 at the infection sites of host cells during Cryptosporidium invasion.
    Lin JJ
    Parasitology research 99.1 (2006 Jun): 45-54.

    Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edge.
    Hitchcock-DeGregori SE
    Journal of cell science 115.Pt 23 (2002 Dec 1): 4649-60.

    Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells.
    Morgan KG
    American journal of physiology. Cell physiology 300.6 (2011 Jun): C1356-65.

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