CH291

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$40.00
SKU: CH291
View product citations for antibody CH291 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: tropomyosin
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Lin, J.J.-C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Broad species, Chicken, Human
Depositors Notes:
Antigen Molecular Weight: Apparent: 39 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: TPM1
Immunogen: Purified chicken cardiac muscle tropomyosin
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: P04268 
Immunogen Sequence: Full length protein
Entrez Gene ID: 396366 
Additional Characterization:
Antibody Registry ID: AB_10807224 
Additional Information: CH291 antibody reacts with chicken cardiac alpha-tropomyosin and skeletal alpha-tropomyosin but not with other isoforms of tropomyosin, including skeletal beta-tropomyosin. CH291 interferes with the binding of tropomyosin to actin. CH291 is specific to ?-tropomyosin in the presence of ?-tropomyosin [PMID: 23712688].
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CH291 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product CH291)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FB References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    FB References
    All References

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