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$45.00 to $425.00
SKU: CH291
View product citations for antibody CH291 on CiteAb

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Available: 37

DSHB Data Sheet

Catalog Fields

Antigen: Tropomyosin, alpha-1 chain (striated muscle)
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Lin, J.J.-C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Broad species, Chicken, Human
Depositors Notes:
Antigen Molecular Weight: Apparent: 39 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: TPM1
Immunogen: Purified chicken cardiac muscle tropomyosin
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: P04268 
Immunogen Sequence: Full length protein
Entrez Gene ID: 396366 
Additional Characterization:  
Antibody Registry ID: AB_10807224 
Additional Information: CH291 antibody reacts with chicken cardiac alpha-tropomyosin and skeletal alpha-tropomyosin but not with other isoforms of tropomyosin, including skeletal beta-tropomyosin. CH291 interferes with the binding of tropomyosin to actin. CH291 is specific to ?-tropomyosin in the presence of ?-tropomyosin [PMID: 23712688].
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CH291 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product CH291)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FB References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    FB References
    All References

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