Antigen: creatine kinase
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Morris, G.E.
Host Species: mouse
Depositors Institution: Centre for Inherited Neuromuscular Disease
Positive Tested Species Reactivity: Chicken, Human, Rabbit, Torpedo
Depositors Notes: This antibody does not work with immunoprecipitation. Binds with rabbit brain, and chicken muscle.
Antigen Molecular Weight: 43 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Alternate Gene Names: CKMM
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Epitope Location or Sequence: 30-89 of chick M-CK with K39 essential
Uniprot ID: P06732
Antibody Registry ID: AB_1553659
Additional Information: RRID:AB_1553659
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CK-2A7 was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product CK-2A7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).