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SKU: CK-5D11
View product citations for antibody CK-5D11 on CiteAb

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Available: 8

DSHB Data Sheet

Catalog Fields

Antigen: creatine kinase
Hybridoma Cells Available: Yes
Antigen Species: human
Depositor: Morris, G.E.
Isotype: MIgG1
Depositors Institution: Centre for Inherited Neuromuscular Disease
Host Species: mouse
Depositors Notes: This antibody does not Western Blot, itæcaptures native MM-CK from muscle extract. It does not work with rabbits, rats, pigs, or chicken.
Positive Tested Species Reactivity: Human
Gene: CKM
Antigen Molecular Weight: 43 kDa
Alternate Gene Names: CKMM
Uniprot ID: P06732 
Clonality: Monoclonal
Entrez Gene ID: 1158 
Epitope Mapped: No
Antibody Registry ID: AB_1553661 
Recommended Applications: Immunofluorescence, Immunoprecipitation
Additional Information: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. RRID:AB_1553661
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CK-5D11 was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product CK-5D11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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