CK-JAC(6F11)

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$40.00
SKU: CK-JAC(6F11)
View product citations for antibody CK-JAC(6F11) on CiteAb

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Available: 38

DSHB Data Sheet

Catalog Fields

Antigen: creatine kinase
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Morris, G.E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Centre for Inherited Neuromuscular Disease
Positive Tested Species Reactivity: Chicken, Fish, Human, Lobster, Mouse, Pig, Pigeon, Quail, Rabbit, Rat, Torpedo
Depositors Notes: This antibody does not work with immunoprecipitation.
Antigen Molecular Weight: 43 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CKM
Immunogen:
Alternate Gene Names: CKMM
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: E182-M206 of chick M-CK
Uniprot ID: P06732 
Immunogen Sequence:
Entrez Gene ID: 1158 
Antibody Registry ID: AB_2618117 
Additional Information: RRID:AB_2618117
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CK-JAC(6F11) was deposited to the DSHB by Morris, G.E. (DSHB Hybridoma Product CK-JAC(6F11))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Monoclonal antibody studies suggest a catalytic site at the interface between domains in creatine kinase.
    Cartwright AJ
    Biochimica et biophysica acta 1039.3 (1990 Jul 6): 318-22.

    All References

    Monoclonal antibody studies suggest a catalytic site at the interface between domains in creatine kinase.
    Cartwright AJ
    Biochimica et biophysica acta 1039.3 (1990 Jul 6): 318-22.

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