CPS 10E8
Catalog Fields
Clone ID/Product Name: CPS 10E8
Available to For-Profits: Yes
Alternate Antibody Name: Anti-CPS (F. tularensis) [10E8]
Gene Symbol:
Ab Isotype: MIgM
Gene Name:
Clonality: Monoclonal
Immunogen:
Clone:
Immunogen Sequence:
Myeloma Strain:
Epitope Mapped: No
Antigen Name: Francisella tularensis capsule
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 11/17/2022
Antigen Molecular Weight:
Depositor: Apicella, M.A.
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: F. tularensis
Depositor Notes:
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity:
Additional Information: RRID: AB_3665182
Recommended Applications: ELISA, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPS 10E8 was deposited to the DSHB by Apicella, M.A. (DSHB Hybridoma Product CPS 10E8)
Storage and Handling Recommendations
Although many cell products are maintained at 4?C for years without loss of activity, shelf-life at 4?C is highly variable. For immediate use, short term storage at 4?C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 µl for freezing at -20?C or -80?C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 µg/ml. For western blots, the recommended concentration range is 0.2-0.5 µg/ml.
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