CPTC-AKR1B1-1

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SKU: CPTC-AKR1B1-1-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: CPTC-AKR1B1-1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: AKR1B1
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2273742 
Uniprot ID: P15121 
RRID:  
Entrez Gene ID: 231 
Clonality: Monoclonal
Immunogen: Recombinant Full Length Protein
Clone:
Immunogen Sequence: Full-length
Myeloma Strain: P3x63Ag8.653
Epitope Mapped: No
Antigen Name: Aldo-keto Reductase Family 1 Member B1
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 10/21/2008
Antigen Molecular Weight: 36.1 kDa
Depositor: Clinical Proteomics Technologies for Cancer
Antigen Sequence:
Depositor Institution: National Cancer Institute
Antigen Species: Human
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabeticcomplications by catalyzing the reduction of glucose to sorbitol. There are a few putativepseudogenes for this gene, and one of them has been confirmed and mapped to chromosome 3.It functions to catalyze the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
Predicted Species Reactivity:  
Recommended Applications: ELISA, FFPE, Microarray, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-AKR1B1-1 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-AKR1B1-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

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